| Coagulation factor VII is synthesized in the liver and circulates in the plasma where it binds to tissue factor (TF) and is rapidly converted into VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and also has important coagulation-independent functions such as angiogenesis. The predominant splicing variant of Factor VII in normal liver corresponds to the 444aa precursor. After cleavage of a signal peptide (residues 1-38), the mature chain can be further processed into the light chain (residues 39-190) and the heavy chain (residues 191-444). |
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| Catalog # | F0015-02 |
| Applications | Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Direct ELISA. Other applications have not been tested. |
| Recommended Dilutions | Western Blot: 1ug/ml detected recombinant human Factor VII. |
| Immunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with recombinant human Factor VII. |
| Immunocytochemistry: 8-25ug/ml using immersion fixed PHA-stimulated human peripheral blood mononuclear cells (PBMCs). |
| Optimal dilutions to be determined by the researcher. |
| Hybridoma | NS0 myeloma cells with spleen cells from Balb/c mice. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 7H85 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.5mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Recombinant protein corresponding to aa39-444 from human Factor VII expressed in NSO cells (NP_062562). Species Sequence Homology: canine - 76%, mouse - 71%, rat - 70%, chicken - 57% |
| Specificity | Recognizes human Factor VII. Does not crossreact with recombinant human Kallikrein-1, -2, -3, -4, -5, -7, -8, -9, -10, -11, -12, -13, -14, -15, -B1, recombinant human HGFA, recombinant human Factor X, recombinant human Factor XI, recombinant human Thrombin, recombinant human uPA-1 or recombinant human PROC. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
