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F4210-14P Sheep Anti-Fibroblast Growth Factor, Basic (FGFb, BFGF, Fibroblast Growth Factor 2, FGF2, Heparin-binding Growth Factor 2, HBGF-2) (Not for Export EU)

Specifications
References
Clone Type
Polyclonal
Host
Sheep
Source
Human
Isotype
IgG
Grade
Serum
Applications
IHC WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Fibroblast growth factors (FGFs), a heparin binding growth factor, exhibit widespread mitogenic and neurotrophic activities in a variety of different cells including mesenchymal, neuroectodermal and endothelial cells. aFGF (FGF-1) and bFGF (FGF-2) are present in relatively high levels in CNS. aFGF is expressed by a subset of neuronal populations, while bFGF is expressed by astrocytes, both lack signal peptides. Human bFGF is a 17.2kD protein containing 155 amino acid residues. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. Subunit: Monomer. Interacts with CSPG4 and FGFBP1. Found in a complex with FGFBP1, FGF1 and FGF2. This protein binds heparin more strongly than does aFGF. Belongs to the heparin-binding growth factors family.

Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000-1:2000 Immunohistochemistry: 1:1000-1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant human basic FGF.
Form
Supplied as a lyophilized powder. Reconstitute in 100ul of sterile ddH2O.
Purity
Serum
Specificity
Recognizes human Basic Fibroblast Growth Factor. Species Crossreactivity: mouse and rat
References
1. Abraham, et al. (1986) Science. 233(4763):545-8 ; 2. Kurokawa, et al. (1987) FEBS Lett. 213(1):189-94 ; 3. Bieger, et al., (1995) J Neurochem 64, 1521-7 ; 4. Takayama, et al., (1995)Nat Med 1, 53-8. ; 5. Eckenstein (1994) J Neurobiol 25, 1467-80. (1994) ; 6. Abraham, et al., (1986) Embo J 5, 2523-8 ; 7. English, et al., (1995)Dev Biol 169, 57-64.
USBio References
No references available
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