FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated, heparin-binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland, liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4.
Recombinant Mouse basic FGF (FGF-2) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 146aa and having a molecular mass of 16.32kD.
Amino Acid Sequence
PALPEDGGAA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHV KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTEE CFFFERLESN NYNTYRSRKY SSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
Biological Activity
Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is ≤ 1ng/ml, corresponding to a specific activity of ≥1x10e6 units/mg.
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
Recombinant, E. coli
Form
Supplied as a lyophilized powder from PBS, pH 7.5. Reconstitute with sterile ddH2O, 0.1% HSA or BSA to ≥0.1mg/ml, which can then be further diluted to other aqueous solutions.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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