FGF-BP, also known as 17kD heparin-binding growth factor binding protein (HBp17) is a secreted, low affinity heparin binding protein. It binds reversibly to FGF-1, -2, -7, -10, and -22 and functions to release the FGFs from the extracellular matrix for interactions with FGF receptors. Human FGF-BP shares 66% amino acid sequence homology with the mouse protein.
Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 0.1ug/ml. The detection limit is ~1ng/lane under non-reducing and reducing conditions. ELISA (Direct): 0.5-1ug/ml. The detection limit is ~0.5ng/well. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Lys34-Cys234 from human FGF-BP expressed in E. coli.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Fibroblast Growth Factor Binding Protein. Shows less than 5% crossreactivity with recombinant rat FGF-BP when tested in ELISA and Western Blot.
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