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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E

Fluorescein is a fluorophore commonly used to label proteins-protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
Catalog #	F5200-09A
Applications	Suitable for use in ELISA. Other applications not tested
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	9L257
Host	Mouse
Concentration	~5mg/ml
Form	Supplied as a liquid in 10mM phosphate, pH 7.4, 150mM sodium chloride, 0.1% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Fluorescein isothiocyanate (FITC).
Specificity	Recognizes Fluorescein Isothiocyanate.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.