Fluorescein isothiocyanate (FITC) is a small fluoresent molecule that is commonly conjugated to antibodies for immunofluorescent cell labeling. In conjunction with anti-FITC antibodies, it can also be used as an epitope tag for detection and signal amplification.
Applications
Suitable for use in Flow Cytometry and ELISA. Other applications not tested.
Recommended Dilutions
ELISA: 0.5ug/ml Optimal dilutions to be determined by researcher.
Hybridoma
Spleen cells from immunized LOU/c rats were fused with cells of the IR983F rat myeloma cell line.
Recommended Secondary Antibodies
S7973-96B: Streptavidin (FITC)
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
FITC conjugated mouse immunoglobulin.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide and 50% glycerol. Labeled with Biotin.
Purity
Purified by affinity chromatography.
Specificity
Recognizes mouse fluorescein isothiocyanate. Avidity against FITC conjugated BSA is 2 x 10e10M-1.This antibody binds to both Free FITC and FITC conjugated to proteins and may be valuable for amplification of FITC based immunostaining.
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