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You are here:Home » Antibodies » Abs to Epitope Tags » Anti -Fluorescein Isothiocyanate (FITC) (Biotin)

Anti -Fluorescein Isothiocyanate (FITC) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E FC
Fluorescein isothiocyanate (FITC) is a small uoresent molecule that is commonly conjugated to antibodies for immuno uorescent cell labeling. In conjunction with anti-FITC antibodies, it can also be used as an epitope tag for detection and signal ampli cation.
Catalog #F5250-11C
ApplicationsSuitable for use in Flow Cytometry and ELISA. Other applications not tested.
Recommended DilutionsELISA: 0.5ug/ml
Optimal dilutions to be determined by researcher.
HybridomaSpleen cells from immunized LOU/c rats were fused with cells of the IR983F rat myeloma cell line.
Recommended Secondary AntibodiesS7973-96B: Streptavidin (FITC)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgM
Clone No3H2148
HostRat
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide and 50% glycerol. Labeled with Biotin.
PurityPurified by affinity chromatography.
ImmunogenFITC conjugated mouse immunoglobulin.
SpecificityRecognizes mouse fluorescein isothiocyanate. Avidity against FITC conjugated BSA is 2 x 10e10M-1.This antibody binds to both Free FITC and FITC conjugated to proteins and may be valuable for amplification of FITC based immunostaining.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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