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Anti -Fluorescein Isothiocyanate (FITC) (Biotin)
Anti -Fluorescein Isothiocyanate (FITC) (Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rat |
Affinity Purified |
E FC |
|
| Fluorescein isothiocyanate (FITC) is a small uoresent molecule that is commonly conjugated to antibodies for immuno uorescent cell labeling. In conjunction with anti-FITC antibodies, it can also be used as an epitope tag for detection and signal ampli cation. | | | Catalog # | F5250-11C | | Applications | Suitable for use in Flow Cytometry and ELISA. Other applications not tested. | | Recommended Dilutions | ELISA: 0.5ug/ml | | Optimal dilutions to be determined by researcher. | | Hybridoma | Spleen cells from immunized LOU/c rats were fused with cells of the IR983F rat myeloma cell line. | | Recommended Secondary Antibodies | S7973-96B: Streptavidin (FITC) | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Monoclonal | | Isotype | IgM | | Clone No | 3H2148 | | Host | Rat | | Source | Mouse | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide and 50% glycerol. Labeled with Biotin. | | Purity | Purified by affinity chromatography. | | Immunogen | FITC conjugated mouse immunoglobulin. | | Specificity | Recognizes mouse fluorescein isothiocyanate. Avidity against FITC conjugated BSA is 2 x 10e10M-1.This antibody binds to both Free FITC and FITC conjugated to proteins and may be valuable for amplification of FITC based immunostaining. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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