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You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -GLi1 (Glioma-associated Oncogene, Kruppel (Kr) Zinc Finger Protein)

Anti -GLi1 (Glioma-associated Oncogene, Kruppel (Kr) Zinc Finger Protein)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
GLI was first identified as a gene amplified in a malignant glioma (1) and can transform primary cells in cooperation with adenovirus E1A (2). GLI belongs to the Kruppel family of zinc finger proteins and there are three GLI proteins in mammals: GLI1, GLI2 and GLI3 (3). GLI proteins, similar to their Drosophila homolog Ci (Cubitus interruptus), function as transcription factors activated by the Hedgehog signaling pathway which plays an important role in animal development and is aberrantly activated in many types of cancers (4,5).
GLI1 itself is a transcriptional target of the Hedgehog signaling pathway (6-8) and is used as a marker of Hedgehog signaling activation in cancer research (9,10).
Catalog #G2035-51
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionImmunoprecipitation: 1:50
Western Blot: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.
PurityPurified by Protein A and immunoaffinity chromatography.
ImmunogenSynthetic peptide (KLH-coupled) corresponding to residues surrounding Gly420 of human GLI1.
SpecificityDetects endogenous levels of total human GLI1 protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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