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G2035-51 Rabbit Anti-GLi1 (Glioma-associated Oncogene, Kruppel (Kr) Zinc Finger Protein)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P08151
Isotype
IgG
Grade
Affinity Purified
Applications
IP WB
Crossreactivity
Hu
Gene ID
2735
Shipping Temp
Blue Ice
Storage Temp
-20°C

GLI was first identified as a gene amplified in a malignant glioma (1) and can transform primary cells in cooperation with adenovirus E1A (2). GLI belongs to the Kruppel family of zinc finger proteins and there are three GLI proteins in mammals: GLI1, GLI2 and GLI3 (3). GLI proteins, similar to their Drosophila homolog Ci (Cubitus interruptus), function as transcription factors activated by the Hedgehog signaling pathway which plays an important role in animal development and is aberrantly activated in many types of cancers (4,5). GLI1 itself is a transcriptional target of the Hedgehog signaling pathway (6-8) and is used as a marker of Hedgehog signaling activation in cancer research (9,10).

Applications
Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended Dilution
Immunoprecipitation: 1:50 Western Blot: 1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to residues surrounding Gly420 of human GLI1.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Detects endogenous levels of total human GLI1 protein.
References
1 Kinzler, K.W. et al. (1987) Science 236, 70-73. 2 Ruppert, J.M. et al. (1991) Mol. Cell Biol. 11, 1724-1728. 3 Kinzler, K.W. et al. (1988) Nature 332, 371-374. 4 Ingham, P.W. and McMahon, A.P. (2001) Genes Dev. 15, 3059-3087. 5 McMahon, A.P. et al. (2003) Curr. Top. Dev. Biol. 53, 1-114. 6 Lee, J. et al. (1997) Development 124, 2537-2552. 7 Dahmane, N. et al. (1997) Nature 389, 876-881. 8 Dai, P. et al. (1999) J. Biol. Chem. 274, 8143-8152. 9 Watkins, D.N. et al. (2003) Nature 422, 313-317. 10 Karhadkar, S.S. et al. (2004) Nature 431, 707-712,
USBio References
No references available
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