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G3057-11 Rabbit Anti-Glucose Regulated Protein, CT, 75kD (Grp 75) (Not for Export EU)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Serum
Applications
WB
Crossreactivity
Hu Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. One class of stress proteins, termed the Hsp70 family, is comprised of multiple members, all of which bind ATP in vitro, but which are localized within different intracellular compartments. These include: i) Hsc70 (or constitutive form) present within the cytosol/nucleus; ii) Hsp70 (inducible form) present within the cytosol/nucleus/nucleolus; iii) the constitutive glucose-regulated 78kD (or BiP) protein present within the lumen of the endoplasmic reticulum; and iv) the constitutive glucose regulated 75kD protein present within the mitochondrial matrix. Members of the Hsp70 family are thought to funtion as molecular chaperones (2), assisting in the folding of other proteins in various intracellular compartments. Grp75 is localized in the mitochondrial matrix, where, in concert with Hsp60, is thought to participate in the re-folding of proteins translocated into this organelle. Like its E. coli homolog DnaK, Grp75 possesses a cation-dependent ATPase activity thought to be central to its function as a chaperone.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000. Chemiluminescent detection at 1-2 minute exposure. Secondary antibody used is anti-rabbit IgG peroxidase. Optimum dilutions to be determined by researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
C-terminal fragment of human Grp 75 (aa608-627).
Form
Supplied as a liquid.
Purity
Serum
Specificity
Recognizes human Grp 75 Species Crossreactivity: Mouse.
References
1. Wadhwa, R., Kaul, S.C., Mitsui, Y., Cell Struct. Function, 19 : 1-10 (1994) 2. Kim, H.T., Nelson, Clayberger, Sanjanwala, M., Sklar, J., Krensky, A.M., J. Immunol., 154 : 1614-1623 (1995) 3. Mizzen, L.A., Chang, C., Garrels, J.I., Welch, W.J., Biol Chem., 264 : 20664-20675 (1989) 4. Webster, T.J., Naylor, D.J., Hartman, D.J., Hoj, P.B., Hoogenraad, N.J., DNA and Cell Biol., 13 : 1213-1220 (1994)
USBio References
No references available
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