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G3900-15B Mouse Anti-GLUT2 (Solute Carrier Family 2, Facilitated Glucose Transporter Member 2, Glucose Transporter Type 2, Liver, GLUT-2, SLC2A2)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2a
Clone Number
11C101
Grade
Affinity Purified
Applications
FC IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free

Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1- Glut-14, Mol. Wt. 40-80kD) and Sodium glucose co-transporter- 1 (SGLT-1, 662aa; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and c-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Human Glut-2 (GTR2, 524aa, chromosome 3q26.1-q26.3, ~60kD) belongs to the family of solute carrier family 2, member 2 or Slc2a2 or facilitative glucose transporter. Glut-2 likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. It is a multi-pass membrane protein. Primarily expressed in liver, insulin-producing beta cell, small intestine and kidney. Defects in SLC2A2 are the cause of Fanconi-Bickel syndrome (FBS, a rare, autosomal recessive mode and characterized by hepatorenal glycogen accumulation, and impaired utilization of glucose and galactose. Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1).

Applications
Suitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended Dilutions
Western Blot: 1-5ug/ml (Chemiluminescence). Do not heat the samples prior to loading to prevent gluts aggregation. Immunohistochemistry; 5-20ug/ml in 4% paraformaldehyde, 0.15% picric acid for 20 minutes and then permeabilized with 0.1% Triton X-100. Flow Cytometry: ~10ug/ml (10e6 cells) Perform the antibody binding in small volume of ~200ul. Optimal dilutions to be determined by the researcher.
Positive Control
Human Caco-2 cells
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human Glut-2. |Epitope: Extracellular domain.
Form
Supplied as a liquid in PBS, pH 7.5. Preservative free.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human Glut-2.
References
1. Fukumoto, H., et al (1988) Proc. Natl. Acad. Sci. 85, 5434-5438. 2. Thorens, B, et al (1988) Cell, 55, 281- 290. 3. Asano, T et al (1989) Nucleic Acid Res. 17, 6386. 4. Suzue, K., et al (1989) Nucleic Acid Res. 17, 10099.
USBio References
No references available
Conjugates
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