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You are here:Home » Antibodies » Abs to Glucose Transporters » Anti -GLUT 2 (Glucose Transporter, Insulin Regulatable)

Anti -GLUT 2 (Glucose Transporter, Insulin Regulatable)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH IC FC
GLUT 2 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. GLUT 2 is expressed predominantly in liver, intestine, kidney and pancreatic beta-cells. It is a low-affinity glucose transporter that has been suggested to function as a glucose sensor in pancreatic beta-cells and facilitate either glucose uptake or efflux from cells depending on the nutritional state.
Catalog #G3900-15N
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 2.5ug to label 1x10e6 NTERA-2 human human cell line
Immunohistochemistry: 8-25ug/ml using immersion fixed paraffin-embedded sections of human brain (medulla), liver, normal pancreas array and pancreatic cancer tissue
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG2a
Clone No5D1
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 5% trehalose.
PurityPurified by Protein A affinity chromatography
ImmunogenRecombinant protein corresponding to aa1-524 from human GLUT 2 expressed in NS0 cells (P11168)
SpecificityRecognizes human GLUT 2 (Glucose Transporter, Insulin Regulatable) expression on hGLUT 2-transfected NS0 cells, but not on control transfectants. Detects GLUT 2 on the surface of the human intestinal cell line Caco-2 in Flow Cytometry and on fixed cells in Immunocytochemistry. Based on Flow Cytometric tests on transfected cells, does not crossreact with human GLUT 1 or GLUT 3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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