| GLUT 2 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. GLUT 2 is expressed predominantly in liver, intestine, kidney and pancreatic beta-cells. It is a low-affinity glucose transporter that has been suggested to function as a glucose sensor in pancreatic beta-cells and facilitate either glucose uptake or efflux from cells depending on the nutritional state. |
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| Catalog # | G3900-15N |
| Applications | Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested. |
| Recommended Dilutions | Flow Cytometry: 0.1mg/ml; Use 10ul to label 1-2.5x10e5 cells in a total reaction volume 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding a stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. |
| Immunocytochemistry: 10ug/ml in fixed cells. Cells were fixed with PBS, 4% paraformaldehyde, 0.15% picric acid at RT for 20 minutes, and subsequently permeabilized and blocked with PBS, 0.1% Triton X-100, 1% BSA, 10% normal donkey serum at RT for 45 minutes. After blocking, cells were incubated with diluted G3900-15N overnight at 4°C followed by Rhodamine Red coupled anti-mouse IgG at RT in the dark for one hour. Between each step, cells were washed with PBS, 0.1% BSA. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 5D1 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in PBS, 5% trehalose. |
| Purity | Purified by Protein G affinity chromatography. |
| Endotoxin | <0.1EU/ug (LAL) |
| Immunogen | Human GLUT 2-transfected NS0 cells. |
| Specificity | Recognizes human GLUT 2 (Glucose Transporter, Insulin Regulatable) expression on hGLUT 2-transfected NS0 cells, but not on control transfectants. Detects GLUT 2 on the surface of the human intestinal cell line Caco-2 in Flow Cytometry and on fixed cells in Immunocytochemistry. Based on Flow Cytometric tests on transfected cells, does not crossreact with human GLUT 1 or GLUT 3. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
