Suitable for use in ELISA, Western Blotting, Immuoprecipitation. Other applications not tested.

Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL. This antibody identified a major band at about 52kD in rat liver microsomal membranes (2).

ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml.

Immuoprecipitation: use at 5-10 ul neat serum per 100ug of rat brain membranes or 1-10ug affinity pure.

Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

A 12 AA peptide sequence near the C-terminus of rat Glucose Transporter 7 (1). A cysteine has been added at the NH2 terminus for coupling to KLH.

Supplied as a liquid, neat serum, 40% glycerol.

Serum

Rat GT72 sequence is identical in human. Glut-7 and Glut-2 sequences are 68% identical in rats and show similar secondary and tertiary structures. However, Glut-7 contains an extra 6 amino acid (KKMKND) at the C-terminal end. This sequence is very similar to the last 6 amino acids of some of the mircosomal UDP-glucoronosyltransferase isoenzymes (see refs. 1 and other refs. Cited therein). The KK-K-- has been considered motif for the retention of transmembrane proteins (3) and is not found in Gluts 1-5. The crossreactivity of our anti-Glut 7 antibody to other proteins with a endoplasmic reticulum motif and species crossreactivity has not been determined. Antibody crossreactivity in various species is not established. Control peptide, because of its low mol size (<3kD), is not recommended for Western. It should be used in ELISA, Dot Blot and for antibody blocking to confirm specificity of antibodies