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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites B

A distinct step in inter-cellular communication involves termination of synaptic tranmission via the removal of neurotramsitters by specialized transporters. The regulated exocytotoxic release of neurotransmitters in reponse to neural activity requires storage within extracellular vesicles. In the nervous sytem, these vesicles are the synaptic vesicles that are derived from the endosomal compartment, whereas in endocrine cells larger secratory granules, such as the chromaffin granules of adrenal medulla, are derived from the trans golgi networks. Glutamate is the main excitatory neurotransmitter in the brain. To date five glutamate transporters have been cloned: GLAST (EAAT1), GLT1 (EAAT2), EAAC1 (EAAT3), EAAT4, and EAAT5. These transporters are believed to be critical in reducing potentially toxic extracellular concentration of glutamate by rapid uptake into nerve terminals and glial cells.
Catalog #	G3990-05A
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1:500-1:2500.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3H22
Host	Mouse
Concentration	Not Determined
Form	Supplied as a liquid in PBS, 0.05% sodium azide.
Purity	Ascites
Immunogen	L-Glutamic acid (KLH).
Specificity	Recognizes L- Glutamic acid when conjugated with glutaraldehye or immobilized on Sepharose. Crossreacts with Gly-Glu and Asp-Glu. Weak crossreactivity may be observed with D-Glu, L-Gln, L-Asn, b-Ala, Gly, 5-aminovaleric acid, g-aminobutyric acid (GABA) and Gly-Asp
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.