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You are here:Home » Antibodies » Abs to Epitope Tags » Anti -Glutathione S-Transferase (GST) (Biotin)

Anti -Glutathione S-Transferase (GST) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
The 26kD GST (glutathione S-Transferase) domain, encoded by the parasite Schistosoma japonicum, is easily purified by affinity chromatography on immobilized glutathione. For this reason, numerous prokaryotic expression vectors have been developed allowing research to fuse recombinant protein purification. GST is utilized as a fusion protein in protein expression from a range of prokaryotic expression vectors, including the pGEX family of vectors. Antibodies against GST will detect the expressed GST protein or a GST-fusion protein, and can be utilized for immuno-purification of GST-fusion proteins.
Catalog #G8135-02T
ApplicationsSuitable for use in ELISA and Western Blot. The antibody is suitable for detecting the expression level of GST in fusion proteins. Other applications not tested.
Recommended DilutionsELISA: 0.05-0.2ug/ml
Western Blot: 0.1-1ug/ml
Optimal dilutions to be determined by the researcher.
HybridomaSp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No8A169
HostMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 0.02% sodium azide. Labeled with Biotin.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant GST protein (26kD).
SpecificityRecognizes C-terminal, N-terminal, and internal tagged GST fusion proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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