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G8140-11B Mouse Anti-Glyceraldehyde 3-Phosphate Dehydrogenase (GAPD, GAPDH) (HRP)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Swiss Prot
P04406
Conjugate
HRP
Isotype
IgG1
Clone Number
9L274
Grade
Affinity Purified
Applications
E
Shipping Temp
Blue Ice
Storage Temp
-20°C

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. Besides functioning as a glycolytic enzyme in cytoplasm, evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. During the last decade, many findings have been made concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death and age-related neuronal diseases such as Alzheimer's and Huntington's. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kD subunits.

Applications
Suitable for use in ELISA. Other applications not tested
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Hybridoma
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Immunogen
Glyceraldehyde 3-Phosphate Dehydrogenase from rabbit muscle
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% ProClin 300. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes Glyceraldehyde 3-Phosphate Dehydrogenase monomer (36kD) and the dimer form. Does not react with the tetrameric form.
References
1. FEBS Letters, (1992), Volume 300, number 1, 9-12.
USBio References
1. Lugenbiel P., et al. 2018. Inhibition of Histone Deacetylases Induces K+ Channel Remodeling and Action Potential Prolongation in HL-1 Atrial Cardiomyocytes. Cell Physiol Biochem. 49(1):66-77. doi: 10.1159/000492840.
Conjugates
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