Glycine is one of the major inhibitory neurotransmitters in the spinal cord and brain stem. Glycine acts on the strychnine-sensitive glycine receptors, a pentameric Cl-channel, to generate inhibitory postsynaptic potentials. Glycine can modulate excitatory neurotransmission as an obligatory co-agonist with glutamate at NMDA-activated glutamate receptors via binding site on the NMDA receptor, which is distinct from the strychnine-sensitive glycine receptor-binding site. Glycinergic synapses are primarily present throughout the spinal cord, brain stem and cerebellum, as well as in the retina and a few other brain regions. Neurotransmitter action is terminated by re-uptake of glycine via specific high affinity transporter protein located at the plasma membrane of neuron and glial cells. The glycine transporter belongs to a family of Na/Cl-dependent neurotransmitter transporters, predicted to contain as many as 12 transmembrane domains. Glycine receptor exists in 2 forms (GLYT1 and GLYT2). Form 1 has three other known isoform (GLYT1a, GLYT1b and GLYT1c) which may be produced by alternative spicing or promoter usage. Rodent GLYT1a and GLYT1b differ only by 10 amino acid at the N-terminus and expressed from the same gene. Although, the N-terminal part of mouse and rat GLYT1a is identical in mouse and rat, the N-terminus of GLYT1b are significantly different in these two species. The GLYT1c subtype has only been reported in humans. GLYT1a is expressed in CNS and peripheral organs. GLYT1b is localized in the CNS. Both neuron and Glial cells have GLYT1. More recent studies indicate that GLYT1b may not be brain specific. Immunolocalization studies on GLYT2 suggest that GLYT2 is responsible for terminating the neurotransmission at the strychnine synapses.

Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.

Antibody Blocking: 5-10ug per 1ul G8168-21 (antiserum) or per 1ug G8168-23 (affinity purified antibody). ELISA: 50-100ng/well. Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Rat synthetic peptide

Highly purified.

~1mg/ml

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.