Granzyme B, also known as CTLA-1, is a serine protease found in the cytotoxic granules of cytotoxic T lymphocytes and NK cells and in articular chondrocytes. Granzyme B enters target cells through perforin channels and induces caspase-dependent apoptosis. It is upregulated in rheumatoid arthritis and cleaves vitronectin, fibronectin, and laminin. Human Granzyme B is synthesized as a precursor with a signal peptide (aa 1-18), a two amino acid propeptide, and a mature chain (aa 21-247). Mature human Granzyme B shares 69% amino acid sequence identity with mouse Granzyme B.
Applications
Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry, Direct ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 0.5ug/ml NK-92 human natural killer lymphoma cell line. Flow Cytometry: 2.5ug/10^6 NK-92 human natural killer lymphoma cell line fixed with paraformaldehyde and permeabilized with saponin. Immunocytochemistry: 8-25ug/mL Immersion fixed human peripheral blood mononuclear cells. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Gly19-Tyr247 from human Granzyme B, expressed in NS0 cells.
Form
Supplied as a lyophilized powder in PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes human Granzyme B. Shows no crossreactivity with rhGranzyme A, -H, rmGranzyme B, -C, -D, or -G.
TDS
NCBI
UniProt
Tech Support
Customer Service
