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Anti -HAPLN4 (Hyaluronan and Proteoglycan Link Protein 4, Brain Link Protein 2)
Anti -HAPLN4 (Hyaluronan and Proteoglycan Link Protein 4, Brain Link Protein 2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B |
|
| Hyaluronan and Proteoglycan Link Protein 4, (HAPLN4), also known as brain link protein 2, is one of four secreted HAPLN family proteoglycans that bind hyaluronan. HAPLN4 contains one Ig-type C2-set domain and two link domains. It is predominantly expressed in brain and participates in the development of the perineuronal matrix. Human and mouse HAPLN4 share 91% aa sequence identity. | | | Catalog # | H1818-50 | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | Direct ELISA: 0.5-1ug/ml The detection limit is ~5ng/well. | | Western Blot: 1-2ug/ml Using a colorimetric detection system, the detection kimit is ~1ng/lane and ~25ng/lane under non-reducing and reducing conditions respectively. Chemilunescent detection will increase sensitivity by 5-50 fold. | | Optimal dilutions to be determined by the researcher. | | Hybridoma | The fusion of mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human HAPLN4. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 7H33 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.2ml is used, the concentration will be 0.5mg/ml. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | NS0-derived recombinant human HAPLN4. | | Specificity | Recognizes human HAPLN4. Does not react with recombinant human HAPLN1. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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