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H1818-50 Mouse Anti-HAPLN4 (Hyaluronan and Proteoglycan Link Protein 4, Brain Link Protein 2)

Specifications
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
Q86UW8
Isotype
IgG2b
Clone Number
7H33
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Gene ID
404037
Shipping Temp
Blue Ice
Storage Temp
-20°C

Hyaluronan and Proteoglycan Link Protein 4, (HAPLN4), also known as brain link protein 2, is one of four secreted HAPLN family proteoglycans that bind hyaluronan. HAPLN4 contains one Ig-type C2-set domain and two link domains. It is predominantly expressed in brain and participates in the development of the perineuronal matrix. Human and mouse HAPLN4 share 91% aa sequence identity.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Direct ELISA: 0.5-1ug/ml The detection limit is ~5ng/well. Western Blot: 1-2ug/ml Using a colorimetric detection system, the detection kimit is ~1ng/lane and ~25ng/lane under non-reducing and reducing conditions respectively. Chemilunescent detection will increase sensitivity by 5-50 fold. Optimal dilutions to be determined by the researcher.
Hybridoma
The fusion of mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human HAPLN4.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
NS0-derived recombinant human HAPLN4.
Form
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human HAPLN4. Does not react with recombinant human HAPLN1.
Conjugates
Pricing
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