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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -HAPLN4 (Hyaluronan and Proteoglycan Link Protein 4, Brain Link Protein 2)

Anti -HAPLN4 (Hyaluronan and Proteoglycan Link Protein 4, Brain Link Protein 2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Hyaluronan and Proteoglycan Link Protein 4, (HAPLN4), also known as brain link protein 2, is one of four secreted HAPLN family proteoglycans that bind hyaluronan. HAPLN4 contains one Ig-type C2-set domain and two link domains. It is predominantly expressed in brain and participates in the development of the perineuronal matrix. Human and mouse HAPLN4 share 91% aa sequence identity.
Catalog #H1818-50
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionDirect ELISA: 0.5-1ug/ml The detection limit is ~5ng/well.
Western Blot: 1-2ug/ml Using a colorimetric detection system, the detection kimit is ~1ng/lane and ~25ng/lane under non-reducing and reducing conditions respectively. Chemilunescent detection will increase sensitivity by 5-50 fold.
Optimal dilutions to be determined by the researcher.
HybridomaThe fusion of mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human HAPLN4.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No7H33
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.2ml is used, the concentration will be 0.5mg/ml.
PurityPurified by Protein G affinity chromatography.
ImmunogenNS0-derived recombinant human HAPLN4.
SpecificityRecognizes human HAPLN4. Does not react with recombinant human HAPLN1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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