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H1827-31D Rabbit Anti-HCN1 pore (Hyperpolarization-activated Cyclic Nucleotide-gated channel Pore)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

HCN channels pass both Na+ and K+ with relative permeability ratio of ~0.15 to 0.25. The primary sequence of HCN pore region is clearly related to that of highly selective K+ channels. In contrast to nonselective CNG or Na+-channels, both K+ and HCN channels contain a GYG motif in the pore. By affecting the spatial coordination of the GYG sequence in the tetrameric channel complex, other channel domains could control the structural rigidity of the pore and thereby determine whether only K+ or also other monovalent are allowed to permeate.

Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilutions
Western Blot: 1-10ug/ml using ECL. ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. Optimal dilutions to be determined by the researcher.
Control Peptide
H1827-31F: HCN1 pore, Human (Hyperpolarization-activated Cyclic Nucleotide-gated channel Pore)
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues aa225-247 near the pore region of human HCN1. Species Sequence Homology: HCN1: mouse, rat and rabbit; 100%; HCN2: mouse, rat and human; 86%; HCN3: mouse; 95%, rat; 90%; HCN4: rat and human; 95%.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human HCN1 pore. The pore region of HCN1 is very conserved among various HCNs.
References
1. Santoro B et al (1997) PNAS 94, 14815-14820. 2. Santoro B et al (1998) Cell 93, 717-729. 3. Steven DR et al (2001) Nature 413, 631-635. 4. Waigner BJ et al (2001) Nature 411, 825-810. 5. Ludwig A et al (1998) Nature 393, 587-591. 6. Biel M et al (2002) Trend Cardiovasc. Med. 12, 206-213
USBio References
No references available
Conjugates
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