| Hepcidin (Hepc, hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins. Hepcidin (unprocessed, proprotein in mouse 83aa, rat/human 84aa) are almost exclusively produced in liver. Human hepcidin is produced from 84aa precursor, including a putative 24aa signal peptide. The secreted form of hepcidin in blood and urine is consists of C-terminal 20, 22 or 25aa residues. In humans, 20aa and 25aa appears to be the major HEPC secreted peptides with antimicrobial activities. The three secreted HEPC alternatively spliced HEPC peptides differ at the N-terminus. The link between hepcidins and iron metabolism is that hepcidin expression is abolished in mice exhibiting iron-overload due to the targeted disruption of USF2 (upstream stimulatory factor 2) gene resembling the situation in hfe-/- mice. The human gene is located at chromosome 19, in close proximity with Usf2 gene. Hepcidin levels are increased in iron loading and in beta-2 microglobulin knockout mice. Hepcidins are devoid of IRE. Like other antimicrobial peptides, hepcidin is upregulated by lipopolysaccharides (LPS). |
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| Catalog # | H2008-72 |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 1-10ug/ml (ECL). |
| ELISA: 1:100,000 using 50-100ng control peptide/well. |
| Optimal dilutions to be determined by the researcher. |
| Control Peptide | H2008-73: Hepcidin 25, Mouse, Human, Control Peptide (Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide) |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Mouse |
| Concentration | ~1mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder in PBS, 0.1% BSA. Reconstitute in 100ul sterile PBS |
| Purity | Purified by immunoaffinity chromatography |
| Immunogen | Synthetic peptides consisting of 7aa sequence from the N-terminus of mature human and mouse 25aa HEPC, conjugated to KLH. |
| Specificity | Recognizes mouse/humanHepcidin 25. The mouse sequences from mouse/rat and human differ by just 1aa in 25aa mature HEPC. The sequence is truncated by 5aa in the 20aa major secreted HEPC. No significant crossreactivity with 20aa HEPC peptide. May crossreacts with the unprocessed larger 60aa pro-HEPC if present in the samples or tissues. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
