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H7500-01 Rabbit Anti-HS1, phosphorylated (Tyr397) (Hematopoietic Specific Protein)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P14317
Isotype
IgG
Grade
Affinity Purified
Applications
IP WB
Crossreactivity
Hu
Gene ID
3059
Shipping Temp
Blue Ice
Storage Temp
-20°C

HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCgamma1 (6). HS1 also plays an important role in platelet activation (7). Tyr397 of HS1 protein is rapidly phosphorylated by Syk and/or Lyn kinases following immune receptor stimulation and thrombin-mediated platelet stimulation. This phosphorylation is an important step in cytoskeletal rearrangement and signaling complex formation (6-10).

Applications
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Tyr397 of human HS1. Uniprot Accession: P14317
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity
Purified by peptide affinity chromatography.
Specificity
Detects the endogenous level of human HS1 protein when phosphorylated at Tyr397.
References
(1) Kitamura, D. et al. (1989) Nucleic Acids Res 17, 9367–79. (2) Kitamura, D. et al. (1995) Biochem Biophys Res Com-mun 208, 1137–46. (3) Suzuki, H. et al. (1997) J Immunol 159, 5881–8. (4) Hata, D. et al. (1994) Immunol Lett 40, 65–71. (5) Yamanashi, Y. et al. (1993) Proc Natl Acad Sci USA 90, 3631–5. (6) Gomez, T.S. et al. (2006) Immunity 24, 741–52. (7) Kahner, B.N. et al. (2007) Blood (epub ahead of print) (8) Yamanashi, Y. et al. (1997) J Exp Med 185, 1387–92. (9) Hao, J.J. et al. (2004) J Biol Chem 279, 33413–20. (10) Brunati, A.M. et al. (2005) J Biol Chem 280, 21029–35.
USBio References
No references available
Conjugates
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