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Specifications

Hyaluronidase catalyzes the random hydrolysis of 1,4-linkages between 2-acetamido-2-deoxy-beta-D-glucose and D-glucose residues in hyaluronate. Hyaluronate 4-glycanohydrolase (EC 3.2.1.35) catalyzes the depolymerization of mucopolysaccharides, hyaluronic acid, and the chondroitin sulfates A and C. The enzyme is widely distributed in animal tissues but is found in great concentrations in the bovine and ovine testes. It is also produced by a number of bacteria. Hyaluronidase from bovine testes has a molecular weight of 55,000. Purified hyaluronidase is used clinically for the intradermal administration of large volumes of fluid when intravenous injections are contraindicated. The enzyme is administered prior to or simultaneously with the fluid and it facilitates absorption of the fluid.
Catalog #	H7981
Activity (USP/NF units/mg dw)	3000u/mg
Absorbance (A280@mg/ml)	~0.85
Unit Definition	One unit is based on the change in absorbency (turbidity) at 540nm of an internal standard.
Quality Control	SDS-PAGE
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Prepare fresh before use..
Certificate of Origin	The raw animal product (bovine glands, organs and tissue) used in the manufacturing of Hyaluronidase is derived from materials of USDA-approved origin. It was collected in USDA or equivalent approved facilities and inspected to be free of disease.
CAS Number	37326-33-3
Source	Bovine testes
Purity	Chromatographically purified
Form	Supplied as a dialyzed, lyophilized powder
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	Bovine testes, Hyaluronate 4-glycanohydrolase, EC=3.2.1.36