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Anti -Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)
Anti -Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Highly Purified |
E B |
|
| Catalog # | I0800-08Q | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:8 | | Optimal dilutions to be determined by the researcher. | | Control Peptide | I0800-08Q1: Inducible Nitric Oxide Synthase, Control Peptide (iNOS, iNOS 2, mNOS); 150nM. Reconstitute with 600ul stock antibody solution for blocked antibody control. | | Recommended Positive Control | A-172 cells | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, TBS/Tween®-20, 1% normal goat serum. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a lyophilized powder. Reconstitution: Reconstitute with 5ml of sterile 40-50% glycerol, TBS/Tween®-20, 1% normal goat serum. | | Purity | Purified by precipitation, dialysis and chromatography. | | Immunogen | Peptide analog of the carboxyl terminal of hiNOS covalently attached onto a carrier protein. | | Specificity | Recognizes hiNOS (1137-1153). The reactivity of rhiNOS (Type 2) is ~50%. Does not react to rhnNOS (Type 1) or rheNOS (Type 3). Has been found to stain specifically hiNOS in Western immunoblots of A-172 cells induced to produce hiNOS. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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