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You are here:Home » Antibodies » Abs to Nitric Oxide » Anti -Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)

Anti -Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B
Catalog #I0800-08Q
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:8
Optimal dilutions to be determined by the researcher.
Control PeptideI0800-08Q1: Inducible Nitric Oxide Synthase, Control Peptide (iNOS, iNOS 2, mNOS); 150nM. Reconstitute with 600ul stock antibody solution for blocked antibody control.
Recommended Positive ControlA-172 cells
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, TBS/Tween®-20, 1% normal goat serum. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder. Reconstitution: Reconstitute with 5ml of sterile 40-50% glycerol, TBS/Tween®-20, 1% normal goat serum.
PurityPurified by precipitation, dialysis and chromatography.
ImmunogenPeptide analog of the carboxyl terminal of hiNOS covalently attached onto a carrier protein.
SpecificityRecognizes hiNOS (1137-1153). The reactivity of rhiNOS (Type 2) is ~50%. Does not react to rhnNOS (Type 1) or rheNOS (Type 3). Has been found to stain specifically hiNOS in Western immunoblots of A-172 cells induced to produce hiNOS.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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