Suitable for use in ELISA and Western Blot. Other applications not tested.

Western Blot: 1:8 Optimal dilutions to be determined by the researcher.

I0800-08Q1: Inducible Nitric Oxide Synthase, Control Peptide (iNOS, iNOS 2, mNOS); 150nM. Reconstitute with 600ul stock antibody solution for blocked antibody control.

A-172 cells

Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, TBS/Tween®-20, 1% normal goat serum. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Peptide analog of the carboxyl terminal of hiNOS covalently attached onto a carrier protein.

Supplied as a lyophilized powder. Reconstitution: Reconstitute with 5ml of sterile 40-50% glycerol, TBS/Tween®-20, 1% normal goat serum.

Purified by precipitation, dialysis and chromatography.

Recognizes hiNOS (1137-1153). The reactivity of rhiNOS (Type 2) is ~50%. Does not react to rhnNOS (Type 1) or rheNOS (Type 3). Has been found to stain specifically hiNOS in Western immunoblots of A-172 cells induced to produce hiNOS.