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I0800-09A1 Inducible Nitric Oxide Synthase, Recombinant, Rat, Western Blot Control (iNOS, iNOS 2, mNOS) CAS:

Specifications
References
Grade
Purified
Applications
WB
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications
Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium. Unlike typical neurotransmitter, NO is a not stored in synaptic vesicle and does not act on membrane receptors. Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues. NO is synthesized by L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthase (NOS, I-III, mol wt. ~130-160kD). One group of enzyme is constitutive, agonist-triggered, and dependent on Ca2+/Calmodulin and is inhibited by L-arginine analogues (L-NNA, L-NMMA). It is found in endothelium, adrenal glands, brain and platelets. The other principle group is inducible, Ca2+/Calmodulin-independent, and inhibited by NMMA and L-NNA. It has been found in macrophage, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells. Analyses of cDNA clones have identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS-III), and macrophage (mNOS/iNOS/NOS-II). Both nNOS and eNOS are constitutive and the mNOS/iNOS is inducible. Sequence homology among different cloned isoforms is ~ 50%.   Suitable for use in Western Blot as a positive control for I0800-09 & I0800-09B. Other applications not tested.
Recommended Dilution
Western Blot: 10ul/lane. May need to add 5ul of fresh 2x sample buffer per 10ul prior to heating and loading gels. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, rat
Purity
Purified
Form
Supplied as a liquid in SDS-PAGE sample buffer (reduced).
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Lowenstein CJ et al (1992) PNAS 89, 6711, 2. Lyons CR et al (1992) J Biol Chem. 267, 6370, Z3. ie Q-W (1992) Science 256, 225 4. Nunokawa Y et al (1993) BBRC 191, 89
USBio References
No references available
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