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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified B FC IF

Catalog #	I1903-13D
Applications	Suitable for immunomicroscopy and ow cytometry or FACS analysis. Other applications not tested.
Recommended Dilution	Flow Cytometry: 1:50 to 1:200 (~1ug will stain 1x10e6 cells)
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Isotype	F(ab’)2
Host	Goat
Source	Rat
Concentration	~0.5-1mg/ml (UV280)
Form	Supplied as a liquid in PBS, pH 7.2, BSA, 0.05% sodium azide, 40% glycerol. Labeled with R-Phycoerythrin (PE). Absorpance Wavelength
Purity	Purified by immunoaf nity chromatography.
Immunogen	Rat IgG F(c) fragment
Specificity	Assay by IEP resulted in a single precipitin arc against anti-phycoerythrin, anti-goat serum, rat IgG, rat IgG F(c) and rat serum. No reaction was observed against anti-pepsin, anti-goat IgG F(c), rat IgG F(ab’)2 or bovine, equine and human serum proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.