| Catalog # | I1903-36N |
| Applications | Suitable for use in Immunofluorescence, ELISA and Fluorescent Western Blot. Other applications have not been tested. |
| Recommended Dilution | ELISA: 1:10,000 -1:50,000 |
| Immunofluoresence: 1:1000-1:5000 |
| Optimal dilutions to be determined by the researcher. |
| Excitation Wavelength | 495nm |
| Emission Wavelength: 528nm |
| Fluorochrome/Protein Ratio | 3.1 moles FITC per mole of Goat IgG F(ab’)2 |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Polyclonal |
| Isotype | F(ab’)2 |
| Host | Goat |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a powder lyophilized from a solution containing PBS, pH 7.4, 0.01% sodium azide, and 10mg/ml IgG and protease free bovine serum albumin (BSA). Labeled with Fluorescein isothiocyanate (FITC; MW= 390 kD). Reconstitute with 1ml deionized water. |
| Purity | Purified by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. |
| Immunogen | Human IgG F(ab’)2 fragment |
| Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Human IgG, Human IgG F(ab’)2 and Human Serum. No reaction was |
| observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(c) or Bovine, Horse, Mouse or Rat Serum Proteins. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
