Suitable for use in Immunofluorescence, FLISA and Fluorescent Western Blot. Other applications have not been tested.

FLISA: 1:10,000 -1:50,000 Immunofluoresence: 1:1000-1:5000 Optimal dilutions to be determined by the researcher.

495nm

528nm

Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Reconstituted product is stable for 12 months at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Human IgG F(ab’)2 fragment

Supplied as a powder lyophilized from a solution containing PBS, pH 7.4, 0.01% sodium azide, and 10mg/ml IgG and protease free bovine serum albumin (BSA). Labeled with Fluorescein isothiocyanate (FITC; MW= 390 kD). Reconstitute with 1ml deionized water.

Purified by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Human IgG, Human IgG F(ab’)2 and Human Serum. No reaction was|observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(c) or Bovine, Horse, Mouse or Rat Serum Proteins.