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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IF

Influenza viruses are common and widely spread infectious agents. Like many other viruses, influenza virus are constantly undergoing mutations and thereby avoiding the immune system.
Catalog #	I7651-20
Applications	Suitable for use in ELISA (1), Western Blot (1), Immunofluorescence. Other applications not tested.
Recommended Dilution	Western Blot: 1:20 (1)
	Immunofluorescence: 1:1000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	3E9/B2
Host	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Purity	Purified by Protein A/G affinity chromatography from culture supernatant.
Immunogen	Influenza-B virus.
Specificity	Reacts with influenza B virus nucleoprotein. No crossreaction is seen with influenza A infected patient samples or purified influenza A virus. Epitope differs from that of clone 6F135 as determined by inhibition ELISA.
Suitable as capture antibody in combination with clone 6F135 as detecting antibody in sandwich ELISA (1). In Western blotting after SDS-PAGE reacts with influenza B viral nucloeprotein (NP). Reacts with influenza B infected, acetone-fixed Vero cells when diluted 1	1000. Specific binding can be visualized by immunofluorescence using a polyclonal anti-mouse Ig antibody labeled with for example fluorescein.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.