USBio Logo

I7660-25A Rabbit Anti-Insulin

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P01308
Isotype
IgG
Grade
Affinity Purified
Applications
FC IC IF IHC
Crossreactivity
Hu Mo Rt
Gene ID
3630
Shipping Temp
Blue Ice
Storage Temp
-20°C

Glucose homeostasis is regulated by hormones. Elevations in blood glucose levels during feeding stimulates insulin release from pancreatic beta cells through a glucose sensing pathway. Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to its secretion. A- and B-peptides are joined by a disulfide bond to form insulin, and the central part of the precursor molecule is cleaved and released as C-peptide. Insulin stimulates glucose uptake from blood into the skeletal muscle and adipose tissue. Insulin deficiency leads to type 1 diabetes mellitus.

Applications
Suitable for use in Immunofluorescence/Immunocytochemistry, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended Dilutions
Immunofluorescence (IC): 1:100 Immunofluorescence (Frozen): 1:100 Immunohistochemistry (Paraffin): 1:100. Antigen retrieval: heat in citrate buffer. Dilute antibody in TBST/5% normal goat serum. Flow Cytometry (Fixed/Permeabilized): 1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to the sequence of human insulin.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes endogenous levels of total human insulin protein. Species Crossreactivity: mouse and rat.
References
1. Straub, S.G. & Sharp, G.W., Diabetes Metab. Res. Rev. 18: 451–463 (2002). 2. Concannon, P., et al., Nat. Genet. 19: 292–296 (1998).
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved