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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E IH FC IF

Glucose homeostasis is regulated by hormones. Elevations in blood glucose levels during feeding stimulates insulin release from pancreatic beta cells through a glucose sensing pathway (1). Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to its secretion. A- and B-peptides are joined by a disulfide bond to form insulin, and the central part of the precursor molecule is cleaved and released as C-peptide. Insulin stimulates glucose uptake from blood into the skeletal muscle and adipose tissue. Insulin deficiency leads to type 1 diabetes mellitus (2).
Catalog #	I7660-25A
Applications	Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended Dilutions	Immunofluorescence (IC): 1:100
	Immunofluorescence (Frozen): 1:100
	Immunohistochemistry (Paraffin): 1:100. Antigen retrieval: heat in citrate buffer. Dilute antibody in TBST/5% normal goat serum.
	Flow Cytometry: 1:100
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity	Purified by Protein A and peptide affinity chromatography.
Immunogen	Synthetic peptide cooresponding to the sequence of human insulin.
Specificity	Recognizes endogenous levels of total human insulin protein. Species Crossreactivity: mouse and rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.