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You are here:Home » Antibodies » Antibodies-Insulin » Anti -Insulin

Anti -Insulin


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IH IC FC IF
Glucose homeostasis is regulated by hormones. Elevations in blood glucose levels during feeding stimulates insulin release from pancreatic beta cells through a glucose sensing pathway. Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to its secretion. A- and B-peptides are joined by a disulfide bond to form insulin, and the central part of the precursor molecule is cleaved and released as C-peptide. Insulin stimulates glucose uptake from blood into the skeletal muscle and adipose tissue. Insulin deficiency leads to type 1 diabetes mellitus.
Catalog #I7660-25A
ApplicationsSuitable for use in Immunofluorescence/Immunocytochemistry, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended DilutionsImmunofluorescence (IC): 1:100
Immunofluorescence (Frozen): 1:100
Immunohistochemistry (Paraffin): 1:100. Antigen retrieval: heat in citrate buffer. Dilute antibody in TBST/5% normal goat serum.
Flow Cytometry: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
ConcentrationNot Determined
FormSupplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
PurityPurified by Protein A and peptide affinity chromatography.
ImmunogenSynthetic peptide corresponding to the sequence of human insulin [Swiss-Port # P01308].
SpecificityRecognizes endogenous levels of total human insulin protein. Species Crossreactivity: mouse, rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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