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You are here:Home » Antibodies » Abs to Integrin » Anti -Integrin alpha5, beta1 (VLA5, Fibronectin Receptor, Very Late Antigen, CD49)

Anti -Integrin alpha5, beta1 (VLA5, Fibronectin Receptor, Very Late Antigen, CD49)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Ascites B IP FC
The alpha 5 beta 1 integrin mediates cell adhesion and migration on fibronectin, a glycoprotein critical for normal vertebrate embryonic development. Indirect evidence reported to date suggests that this receptor also functions in the deposition of fibronectin matrices.
Catalog #I7661-35Q
Specificity of the antibody was verified by its ability to immunoprecipitate a5b1 heterodimers from 125-I-surface labeled cells, reciprocal depletion of VLA-5 antigen from cell lysate with antiserum against the cytoplasmic domain of a5 integrin subunit, and immunoprecipitation of VLA-5 integrin from cells known to express this integrin. Cell lines tested to express a5b1, include P19 cells, mouse melanoma B16F1 cells, mouse squamous carcoma SCC cells and thymocytes. Characterization by immunoprecipitation was typically analyzed by SDS-PAGE using 6% gels under non-reducing condition
ApplicationsInhibition of cell adhesion to fibronectin: 10mg/ml Note that where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (a4b1) integrin, is involved, inhibition of cell adhesion by BMB5 alone may not be complete.
Immunoprecipitation from lysate of 10e6 cell equivalent: 2mg
Flow cytometry: 10mg/ml Western blot (non-reducing gel)
Storage and Handling Maintain frozen at-20°C in undiluted aliquots for up to 12 months.
Clone TypeMonoclonal
IsotypeIgG3
Clone No2Q963
HostRat
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, containing no preservatives
PurityAscites
ImmunogenThe antibody was prepared by fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells
SpecificityRecognizes mouse integrin VLA-5 (a5b1), a fibronectin receptor.
Species CrossreactivityMouse
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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