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Anti -Integrin alpha5, beta1 (VLA5, Fibronectin Receptor, Very Late Antigen, CD49)
Anti -Integrin alpha5, beta1 (VLA5, Fibronectin Receptor, Very Late Antigen, CD49)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rat |
Ascites |
B IP FC |
|
| The alpha 5 beta 1 integrin mediates cell adhesion and migration on fibronectin, a glycoprotein critical for normal vertebrate embryonic development. Indirect evidence reported to date suggests that this receptor also functions in the deposition of fibronectin matrices. | | | Catalog # | I7661-35Q | | Specificity of the antibody was verified by its ability to immunoprecipitate a5b1 heterodimers from 125-I-surface labeled cells, reciprocal depletion of VLA-5 antigen from cell lysate with antiserum against the cytoplasmic domain of a5 integrin subunit, and immunoprecipitation of VLA-5 integrin from cells known to express this integrin. Cell lines tested to express a5b1, include | P19 cells, mouse melanoma B16F1 cells, mouse squamous carcoma SCC cells and thymocytes. Characterization by immunoprecipitation was typically analyzed by SDS-PAGE using 6% gels under non-reducing condition | | Applications | Inhibition of cell adhesion to fibronectin: 10mg/ml Note that where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (a4b1) integrin, is involved, inhibition of cell adhesion by BMB5 alone may not be complete. | | Immunoprecipitation from lysate of 10e6 cell equivalent: 2mg | | Flow cytometry: 10mg/ml Western blot (non-reducing gel) | | | | Storage and Handling | Maintain frozen at-20°C in undiluted aliquots for up to 12 months. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG3 | | Clone No | 2Q963 | | Host | Rat | | Source | Mouse | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, containing no preservatives | | Purity | Ascites | | Immunogen | The antibody was prepared by fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells | | Specificity | Recognizes mouse integrin VLA-5 (a5b1), a fibronectin receptor. | | Species Crossreactivity | Mouse | | | Important Note | This product is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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