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Interferon alpha 4, Recombinant, Mouse (Interferon alpha-4, IFN-alpha-4, Ifna4)
Interferon alpha 4, Recombinant, Mouse (Interferon alpha-4, IFN-alpha-4, Ifna4)
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| Most mammalian species have multiple IFN-a subtypes. Although the reasons for these multiple subtypes are not fully known, there are clear cell type and temporal differences in their expression. A recent study established a nomenclature for the murine IFN-a subtypes (van Pesch et al. 2004) and determined relative activities of the subtypes with protein quantification by phosphorimaging of metabolically-labeled protein. In this study, Mu IFN-a4 was deemed to have average antiviral activity when compared with the potency of the other subtypes. Mu IFN-a4 was initially cloned by Zwarthoff et al. [(1985) Nuc. Acids Res. 13(3) 791], and has been extensively studied. It is apparently expressed early in viral infection in a protein synthesis independent manner, and its expression is induced by phosphorylation of IRF-3. It may be that Mu IFN-a4, like IFN-b, has a priming function on cells, enabling the expression of other Mu IFN-a subtypes [Reviewed by Mesplède et al. (2003) Autoimmunity 36(8):447 and Asselin-Paturel & Trinchieri (2005) J. Exp. Med. 202(4):461]. Thus, this interferon is among the first observed after viral infection. Intriguingly, while this interferon is expressed in a large variety of cell types, one report suggests that the expression level in dendritic cells is low to non-existent [Barchet et al. (2002) J. Exp. Med. 195(4):507] | | | Catalog # | I7661-75M | | Source | Recombinant corresponding to murine (C57/Bl) DNA expressed in mammalian cells. | | Molecular Weight | ~19.5kD | | Biological Activity | Determined relative to a lab standard of Mu IFN-alpha A which was calibrated to the NIH Murine IFN-a standard (Ga02-901-511). Mouse (L929/EMCV) in this assay the EC50 for IFN is ~5U/ml. | | Endotoxin | ~1EU/ug | | Storage and Stability | Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | Molecular Weight | ~19.5kD | | Source | Mammalian tissue culture media | | Purity | ~95% (Ion exchange, hydrophobic interaction and size exclusion chromatography) | | Concentration | As reported | | Form | Supplied as a liquid in PBS, 0.1% BSA. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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