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I7662-93G Rabbit Anti-IRF8 (Interferon Regulatory Factor 8, IRF-8, Interferon Consensus Sequence-binding Protein, ICSBP, IRF8, ICSBP1)

Specifications
References
Clone Type
Monoclonal
Host
Rabbit
Source
Human
Swiss Prot
Q02556
Isotype
IgG
Clone Number
D20D8
Grade
Purified
Applications
ChIP IP WB
Crossreactivity
Hu Mo
Gene ID
3394
Shipping Temp
Blue Ice
Storage Temp
-20°C

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3g/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7 and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS) and IFN regulatory elements (IRF-E) (2). IRF-8/ICSCP is expressed predominately in hematopoietic cells and is further increased upon treatment with interferon (3,4). IRF-8 can function as a transcription repressor of ICS-containing promoters (4). Expression of IRF-8 can lead to the down-regulation of the anti-apoptotic protein Bcl-2 (5). Originally described as being induced by IFN-y, IRF-8 expression is also elevated by IRF-a as well as IL-12 in NK and T cells (6). IRF-8 deficient mice have enhanced susceptibility to various pathogens and impaired production of interferons, as well as deregulated hematopoiesis that resembles chronic myelogenous leukemia (7,8). IRF-8 also regulates bone metabolism by suppressing osteoclast formation (9).

Applications
Suitable for use in ChIP, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Immunoprecipitation: 1:50 ChIP: 1:25 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues surrounding Gly65 of human IRF-8. Species sequence homology: rat, monkey, xenopus, and bovine (100%).
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity
Purified.
Specificity
Recognizes endogenous levels of total human IRF-8. Species Crossreactivity: mouse.
References
1.Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55. 2.Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58. 3.Driggers, P.H. et al. (1990) Proc Natl Acad Sci U S A 87, 3743-7. 4.Weisz, A. et al. (1992) J Biol Chem 267, 25589-96. 5.Burchert, A. et al. (2004) Blood 103, 3480-9. 6.Lehtonen, A. et al. (2003) Cytokine 24, 81-90. 7.Holtschke, T. et al. (1996) Cell 87, 307-17. 8.Fehr, T. et al. (1997) J Exp Med 185, 921-31. 9.Zhao, B. et al. (2009) Nat Med 15, 1066-71.
USBio References
No references available
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