USBio Logo

I7662-94 Rabbit Anti-Interferon Stimulating Gene 15 (ISG15, 15kD ubiquitin-like modifier GIP2, IFI15, Ubiquitin Cross-reactive Protein, UCRP)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Highly Purified
Applications
E WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C (-20°C Glycerol)
Notes
BSA Free

Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg12, Hub1. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not proteolytically processed or conjugated to other proteins. ISG15 (Interferon Stimulating Gene-15) shows no amino acid sequence homology to cytokines and is synthesized as a precursor that is activated through processing by a thiol protease. ISG15 is secreted by monocytes and lymphocytes. Synthesis is induced in response to IFN-a or IFN-b or IFN-a, but not IFN-g. ISG15 expression is induced also by overexpression of some interferon regulatory factors that have been shown to play a role in the transcriptional regulation of IFN genes. ISG15 is secreted also by cell lines of monocyte (U937 cell line), T-lymphocyte, B-lymphocyte (DAUDI cells), human fibroblasts, and epithelial origins. The induction of terminal differentiation in human melanoma cells is associated, among other things, with alterations in the expression of ISG15. Intracellularly ISG15 has been shown to function as a ubiquitin homologue. It is known also as UCRP (ubiquitin cross-reactive protein). Serpin 2a (spi2a), a member of the serine protease inhibitor (serpin) protein family that is highly induced in macrophages during bacillus Calmette-Guerin infection has been shown to bind ISG15. ISG15 has been shown to modulate immune cell function. It possesses activities of cytokines and induces production of IFN-g. It enhances proliferation and functions of natural killer and LAK cells.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:2000-1:10,000 Western Blot: 1:200-1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, ddH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant human ISG15 protein.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added. Reconstitute with 100ul sterile, sterile 40-50% glycerol, ddH2O.
Purity
Purified by delipidation, salt fractionation and ion exchange chromatography.
Specificity
Recognizes human ISG15. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum.
References
D'Cunha, J. et al. (1996) Immunoregulatory properties of ISG15 interferon-induced cytokine. Proc. Natl, Acad. Sci. USA 93; 211-215.|Potter J.L.et al (1999) Precursor Processing of Pro-ISG15/UCRP, an Interferon--induced Ubiquitin-like Protein. J Biol Chem 274;35, 25061-25068.|Malakhov MP et al (2002) UBP43 (USP18) specifically removes ISG15 from conjugated proteins. J Biol Chem.277(12):9976-81.|Muller, S. , Hoege, C. , Pyrowolakis, G. and Jentsch, S. (2001) SUMO, ubiquitin's mysterious cousin. Nat Rev Mol Cell Biol, 2(3): 202-10.|Liakopoulos D et al. (1998). A novel protein modification pathway related to the ubiquitin system. EMBO J. 15;17(8):2208-14.|Jentsch S, Pyrowolakis G. (2000) Ubiquitin and its kin: how close are the family ties? Trends Cell Biol. 10(8):335-42.
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved