Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 1 alpha (IL-1a)

Anti -Interleukin 1 alpha (IL-1a)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B N
IL-1alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine.
Catalog #I7663-02E
Recommended Secondary ReagentsSheep anti Rabbit IgG (Aff. Pur.): HRP
ApplicationsSuitable for use in ELISA, Western Blot, Neutralization. Other applications not tested.
Recommended DilutionELISA: 0.5ug/ml
Western Blot: 0.1-0.2ug/ml
Neutralization: 0.03-0.05ug/ml to yield one half maximal inhibition (ND50) of mouse IL-1 alpha
Optimal working dilutions to be determined by researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml
FormSupplied as a lyophilized powder in PBS. No preservatives added. Reconstitute with 0.1ml distilled water.
PurityPurified by affinity chromatography.
ImmunogenRecombinant mouse IL-1 alpha.
SpecificitySpecific for mouse interleukin-1 alpha (IL-1 alpha).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links