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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B N

IL-1alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine.
Catalog #	I7663-02E
Recommended Secondary Reagents	Sheep anti Rabbit IgG (Aff. Pur.): HRP
Applications	Suitable for use in ELISA, Western Blot, Neutralization. Other applications not tested.
Recommended Dilution	ELISA: 0.5ug/ml
	Western Blot: 0.1-0.2ug/ml
	Neutralization: 0.03-0.05ug/ml to yield one half maximal inhibition (ND50) of mouse IL-1 alpha
	Optimal working dilutions to be determined by researcher.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	~1mg/ml
Form	Supplied as a lyophilized powder in PBS. No preservatives added. Reconstitute with 0.1ml distilled water.
Purity	Purified by affinity chromatography.
Immunogen	Recombinant mouse IL-1 alpha.
Specificity	Specific for mouse interleukin-1 alpha (IL-1 alpha).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.