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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 1 beta (IL-1b) (Biotin)

Anti -Interleukin 1 beta (IL-1b) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B FC
Catalog #I7663-07A
ApplicationsSuitable for use in Flow Cytometry and Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 0.1-0.2ug/ml. Detection limit for recombinant canine IL-1beta is ~0.5ng/lane under non-reducing and reducing conditions. Shows ~40% cross-reactivity with recombinant feline IL-1beta, 10% cross-reactivity with recombinant human IL-beta, rrmIL-1beta, recombinant equine IL-1 beta, recombinant porcine IL-1beta, and 5% cross-reactivity with rmIL-1beta, recombinant rat IL-1beta, and rcrIL-1beta.
Flow Cytometry: Dilute to 50ug/ml. Use 10ul of dilution to 1-2.5x10e5 cells.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile with 40-50% glycerol, TBS, 0.1% BSA and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceCanine
ConcentrationNot determined.
FormSupplied as a lyophilized powder from PBS and BSA. Labeled with Biotin. Reconstitute with sterile 40-50% glycerol, TBS (20mM Trizma vase, 150mM NaCl), 0.1% BSA.
PurityPurified by affinity chromatography.
ImmunogenE. coli-derived recombinant canine IL-1beta.
SpecificityRecognizes canine Interleukin 1 beta (IL-1b).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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