USBio Logo

I7663-07A Goat Anti-Interleukin 1 beta (IL-1b) (Biotin)

Specifications
Clone Type
Polyclonal
Host
Goat
Source
Canine
Swiss Prot
Q28292
Conjugate
Biotin
Isotype
IgG
Grade
Affinity Purified
Applications
FC WB
Crossreactivity
Ca
Gene ID
403974
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications
Suitable for use in Flow Cytometry and Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 0.1-0.2ug/ml. Detection limit for recombinant canine IL-1beta is ~0.5ng/lane under non-reducing and reducing conditions. Shows ~40% cross-reactivity with recombinant feline IL-1beta, 10% cross-reactivity with recombinant human IL-beta, rrmIL-1beta, recombinant equine IL-1 beta, recombinant porcine IL-1beta, and 5% cross-reactivity with rmIL-1beta, recombinant rat IL-1beta, and rcrIL-1beta. Flow Cytometry: Dilute to 50ug/ml. Use 10ul of dilution to 1-2.5x10e5 cells. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile TBS, 0.1% BSA and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
E. coli-derived recombinant canine IL-1beta, Asp114-Ser265.
Form
Supplied as a lyophilized powder from PBS and BSA. Labeled with Biotin. Reconstitute with sterile TBS (20mM Trizma vase, 150mM NaCl), 0.1% BSA.
Purity
Purified by affinity chromatography.
Specificity
Recognizes canine Interleukin 1 beta (IL-1b).
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved