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Anti -Interleukin 1 beta (IL-1b) (Biotin)
Anti -Interleukin 1 beta (IL-1b) (Biotin)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
B FC |
|
| Catalog # | I7663-07A | | Applications | Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. | | Recommended Dilutions | Western Blot: 0.1-0.2ug/ml. Detection limit for recombinant canine IL-1beta is ~0.5ng/lane under non-reducing and reducing conditions. Shows ~40% cross-reactivity with recombinant feline IL-1beta, 10% cross-reactivity with recombinant human IL-beta, rrmIL-1beta, recombinant equine IL-1 beta, recombinant porcine IL-1beta, and 5% cross-reactivity with rmIL-1beta, recombinant rat IL-1beta, and rcrIL-1beta. | | Flow Cytometry: Dilute to 50ug/ml. Use 10ul of dilution to 1-2.5x10e5 cells. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile with 40-50% glycerol, TBS, 0.1% BSA and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Canine | | Concentration | Not determined. | | Form | Supplied as a lyophilized powder from PBS and BSA. Labeled with Biotin. Reconstitute with sterile 40-50% glycerol, TBS (20mM Trizma vase, 150mM NaCl), 0.1% BSA. | | Purity | Purified by affinity chromatography. | | Immunogen | E. coli-derived recombinant canine IL-1beta. | | Specificity | Recognizes canine Interleukin 1 beta (IL-1b). | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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