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You are here:Home » Antibodies » Abs to Interleukin » Anti -Interleukin 1 beta (IL-1b) (Azide Free)

Anti -Interleukin 1 beta (IL-1b) (Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E N
IL-1 beta is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell matur- ation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stim- ulate the release of prostaglandin and collagenase from syn- ovial cells. IL-1beta is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
Catalog #I7663-11A2
ApplicationsSuitable for use in ELISA and Neutralization. Other applications not tested.
Recommended DilutionSandwich ELISA: 10pg/ml-3ng/ml after zymosan induced inflammation (2). Can be used as the capture reagent in a two-site sandwich ELISA for measuring mouse IL-1b levels using recombinant mouse IL-1b as the standard.
Optimal dilution to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No4H130
HostRat
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS. Azide-free.
PurityPurified by Protein G affinity chromatography.
ImmunogenMouse Interleukin 1 beta .
SpecificityRecognizes both the recombinant and the natural form of mouse IL-1b.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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