Interleukin 5 (IL-5) is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentation and activation of eosinophils. It binds to a receptor complex consisting of one IL-5 specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, and Flow Cytometry. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1ug/ml using recombinant human IL‑5 Immunocytochemistry: 8-25ug/ml using man peripheral blood lymphocytes Flow Cytometry: 2.5ug/ml labels 10e6 cells using Ca2+ionomycin-treated human PBMCs Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
I1904-65V IgG, F(ab’)2 (FITC) Pab RbxMo I1904-66A IgG, F(ab’)2, X-Adsorbed (FITC) ) Pab RbxMo
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Ile20-Ser134 from human IL-5, expressed Sf21 cells.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Purity
Purified by Protein G affinity chromatography from ascites.
Specificity
Recognizes human IL-5. Based on direct ELISA and Western Blot results, this antibody shows no crossreactivity with rmIL-5.
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