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You are here:Home » Antibodies » Antibodies-Interleukin » Anti -Interleukin 6 Receptor (CD126, IL-6R)

Anti -Interleukin 6 Receptor (CD126, IL-6R)


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Clone Host Grade Applications
Monoclonal Rat Affinity Purified IC FC
IL-6 R, also known as CD126, is a transmembrane protein that associates with the signal-transducing subunit gp130 to form a high-affinity receptor complex for IL-6. It is expressed on T cells, activated B cells, and peripheral monocytes. A soluble form of IL-6 R can be released by proteolytic cleavage.
Catalog #I8428-39M
ApplicationsSuitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 2.5ug/10e6 cells using mouse CD3+ splenocytes
Immunocytochemistry: 8-25ug/ml using immersion fixed mouse splenocytes
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
Clone No8H130
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
PurityPurified by Protein G affinity chromatography
ImmunogenRecombinant protein corresponding to aa20-357 from mouse IL-6R expressed in NSO cells (P22272).
SpecificityRecognizes mouse IL-6R. Does not crossreact with rhIL-6R.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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