| IL-6 R, is a transmembrane protein that associates with the signal-transducing subunit gp130 to form a high-affinity receptor complex for IL-6. It is expressed on T cells, activated B cells, and peripheral monocytes. A soluble form of IL-6 R can be released by proteolytic cleavage. |
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| Catalog # | I8428-39M |
| Applications | Suitable for use in Flow Cytometry, ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Flow Cytometry: 25ug/ml; 10ul label 1-2.5 x 10e5 cells in a total reaction volume < 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. |
| Direct ELISA: 0.5-1.0ug/ml; The detection limit for is ~5ng/well. |
| Western Blot: 1-2ug/ml; Using a colorimetric detection system, the detection limit is ~5 ng/lane and ~50 ng/lane under non-reducing and reducing conditions, respectively. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. |
| Optimal dilutions to be determined by the researcher. |
| Hybridoma | Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, NS0-derived, recombinant mouse Interleukin-6 Receptor (rmIL-6 R) extracellular domain. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 8H130 |
| Host | Rat |
| Source | Mouse |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS. |
| Purity | Purified by Protein G affinity chromatography. |
| Endotoxin | < 0.1 EU/ug (LAL) |
| Immunogen | NS0-derived recombinant mouse IL-6R extracellular domain |
| Specificity | Recognizes mouse IL-6R. Does not crossreact with rhIL-6R in direct ELISAs and Western Blots. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
