Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 2.5ug/ml will detect 1ug/lane of human IL-16. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to human IL-16
Form
Supplied as a lyophilized powder in 0.01M PBS, pH 7.2. Reconstitute with sterile ddH2O to ~1mg/ml.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human IL-16. Exhibits no detectable crossreactivity with human albumin serum, bovine albumin serum and other human cytokines or growth factors tested such as IL-1b, IL-7, IL-8, MCAF, TGF-b1, EGF, bFGF, GM-CSF, M-CSF, TNF-a.