Suitable for use in ELISA and Flow Cytometry. Other applications not tested.

Flow Cytometry: 25ug/10e6 using Tramp-C1 cells mouse prostate cancer cell line. For intracellular staining to detect IL-17C, cells must first be fixed and permeabilized using paraformaldehyde and saponin in PBS. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as anti-rat IgG (PE). Optimal dilutions to be determined by the researcher.

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recombinant protein corresponding to His15-Gln194 from mouse Interleukin 17C (IL-17C), expressed in E. coli.

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

Purified by Protein G affinity chromatography from hybridoma culture supernatant.

Recognizes mouse Interleukin 17C (IL-17C).