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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -IRS-4, CT (Insulin Receptor Substrate 4, 160kD Phosphotyrosine Protein, OTTHUMP00000023837, PY160)

Anti -IRS-4, CT (Insulin Receptor Substrate 4, 160kD Phosphotyrosine Protein,
OTTHUMP00000023837, PY160)


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Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH FC
A new member of the IRS family, IRS-4, is a 160kD tyrosine kinase which phosphorylates cellular substrates when it is activated by the binding of insulin. IRS-4 was originally discovered in human embryonic kidney 293 cells. Subcellular fractionation revealed that about 50% of IRS-4 was located in cellular membranes, and immunofluorescence indicated that IRS-4 was concentrated at the plasma membrane. Immunoelectron microscopy conclusively established that a large portion of the IRS-4 was located at the cytoplasmic surface of the plasma membrane in both the unstimulated and insulin-treated states. IRS-4 was found to be associated with two src homology 2 (SH2) domain-containing proteins, phosphatidylinositol 3-kinase and Grb2, the adaptor to the guanine nucleotide exchange factor for Ras.
Catalog #I8700-26B
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:20
Western Blot: 1:1000-1:10,000
Immunoprecipitation: 1:20
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
Clone No9H423
ConcentrationNot Determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
ImmunogenA synthetic peptide corresponding to residues on the C-terminus of human IRS-4.
SpecificityRecognizes human IRS4. Crossreactivity tested in Western Blot only.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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