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Anti -IRS-4, CT (Insulin Receptor Substrate 4, 160kD Phosphotyrosine Protein, OTTHUMP00000023837, PY160)
Anti -IRS-4, CT (Insulin Receptor Substrate 4, 160kD Phosphotyrosine Protein,
OTTHUMP00000023837, PY160)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IP IH FC |
|
| A new member of the IRS family, IRS-4, is a 160kD tyrosine kinase which phosphorylates cellular substrates when it is activated by the binding of insulin. IRS-4 was originally discovered in human embryonic kidney 293 cells. Subcellular fractionation revealed that about 50% of IRS-4 was located in cellular membranes, and immunofluorescence indicated that IRS-4 was concentrated at the plasma membrane. Immunoelectron microscopy conclusively established that a large portion of the IRS-4 was located at the cytoplasmic surface of the plasma membrane in both the unstimulated and insulin-treated states. IRS-4 was found to be associated with two src homology 2 (SH2) domain-containing proteins, phosphatidylinositol 3-kinase and Grb2, the adaptor to the guanine nucleotide exchange factor for Ras. | | | Catalog # | I8700-26B | | Applications | Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | Flow Cytometry: 1:20 | | Western Blot: 1:1000-1:10,000 | | Immunoprecipitation: 1:20 | | Immunohistochemistry: 1:50-1:100 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 9H423 | | Host | Rabbit | | Source | Human | | Concentration | Not Determined | | Form | Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol. | | Purity | Supernatant | | Immunogen | A synthetic peptide corresponding to residues on the C-terminus of human IRS-4. | | Specificity | Recognizes human IRS4. Crossreactivity tested in Western Blot only. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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