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Iso-Sensitest Agar


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Iso-Sensitest Agar is a medium developed specifically for anti-microbial susceptibility tests like the Disc Diffusion Method. This is a well-defined medium containing a stabilized mineral content to avoid antagonism to certain antimicrobials by metal ions. It allows the growth of the great majority of microorganisms without further supplementation. It can also be supplemented for fastidious organisms with 5% whole horse blood or 5% whole horse blood and 20mg/l beta-nicotinamide adenine dinucleotide (NAD). Some mutant strains which are totally dependent on thymine and thymidine for their growth will not grow on Iso-Sensitest Agar which has these two components at very low levels. Care must be taken to recognize these strains.
Catalog #I9090
Certain supplements interefere with anti-microbial activity and tests must be performed to measure their effect. These supplements and the anti-microbial agents affected (in parentheses) include thymidine (trimethoprim), blood (sulfonamides, trimethoprim, aminoglycosides), carbon dioxide (aminoglycosides, erythromycin, lincomycin, tetracycline, novobiocin), cysteine and other sulfhydryl compounds (aminoglycosides), and Vitox/Isovitalex (sulfonamides, trimethoprim).
The addition of lysed horse erthrocytes to the medium is not required when carrying out anti-microbial susceptibility tests with trimethoprim and sulfonamides
Dehydrated AppearanceStraw colored, homogenous, free flowing powder.
Appearance of Prepared MediumStraw colored gel
pHAs reprorted
Microbiological Testing
Escherichia coli ATCC 25922Growth; pale colored colonies
Staphylococcus aureus ATCC 25923Growth; cream colored colonies
Pseudomonas aeruginosa ATCC 27853Growth; straw colored colonies

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