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You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -Jumonji Domain Containing Protein 1B (JMJD1B, JmjC Domain-containing Histone Demethylation Protein 2B, JHDM2B, KDM3B, 5qNCA, Nuclear Protein 5qNCA)

Anti -Jumonji Domain Containing Protein 1B (JMJD1B, JmjC Domain-containing Histone
Demethylation Protein 2B, JHDM2B, KDM3B, 5qNCA, Nuclear Protein 5qNCA)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Serum B IP IF
The methylation state of lysine residues in histone proteins is a major determinant of the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain of several proteins has been shown to catalyze the demethylation of mono-, di-, and trimethyl lysine residues via an oxidative reaction that requires iron and -ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into seven separate families (3). The JMJD1 (Jumonji domain-containing protein 1) family, also known as JHDM2 (JmjC domain-containing histone demethylation protein 2) family, contains four members: hairless (HR), JMJD1A/JHDM2A, JMJD1B/JHDM2B, and JMJD1C/JH- DM2C. Hairless is expressed in the skin and brain and acts as a co-repressor of the thyroid hormone receptor (4-6). Mutations in the hairless gene cause alopecia in both mice and humans (4,5). JMJD1A is expressed in meiotic and post-meiotic male germ cells, contributes to androgen receptor-mediated gene regulation, and is required for spermatogenesis (7-9). It has also been identified as a downstream target of OCT4 and STAT3 and is critical for the regulation of self-renewal in embryonic stem cells (10,11). JMJD1B is a more widely expressed family member and is frequently deleted in myeloid leukemia (12). JMJD1C (also known as TRIP8) is a co-factor of both the androgen and thyroid receptors and has a potential link to autism (13-15). Members of the JMJD1/JHDM2 family have been shown to demethylate mono-methyl and di-methyl histone H3 (Lys9) (3,8).
Catalog #J7876-42B
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunofluorescence (IF-IC). Other applications not tested.
Recommended DilutionWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X
TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Immunofluorescence (IF-IC): 1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10E35
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied in liquid 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and 0.02% sodium azide.
PuritySerum
ImmunogenRecombinant protein derived from the sequence of human JMJD1B.
SpecificityRecognizes human JMJD1B (6A1-1F5). Species crossreactivity: mouse, rat, monkey.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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