Anti -Jumonji Domain Containing Protein 1B (JMJD1B, JmjC Domain-containing Histone
Demethylation Protein 2B, JHDM2B, KDM3B, 5qNCA, Nuclear Protein 5qNCA)
Pricing
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| Clone |
Host |
Grade |
Applications |
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Rabbit |
Affinity Purified |
B IP IF |
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| The methylation state of lysine residues in histone proteins is major determinant for formation of active and inactive regions of the genome and is crucial for proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain of several proteins has been shown to catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and -ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be di- vided into seven separate families (3). The JMJD1 (Jumonji domain-containing protein 1) family, also known as JHDM2 (JmjC domain-containing histone demethylation protein 2) family, contains four members: hairless (HR), JMJD1A/JH- DM2A, JMJD1B/JHDM2B, JMJD1C/JHDM2C. Hairless is expressed in the skin and brain and acts as a co-repressor of the thyroid hormone receptor (4-6). Mutations in the hairless gene cause alopecia in both mice and humans (4,5). JMJD1A is expressed in meiotic and post-meiotic male germ cells, contributes to androgen receptor-mediated gene regulation, and is required for spermatogenesis (7-9). It has also been identified as a downstream target of OCT4 and STAT3 and is critical for the regulation of self-renewal in embryonic stem cells (10,11). JMJD1B is a more widely expressed family member and is frequently deleted in myeloid leukemia (12). JMJD1C (also known as TRIP8) is a co-factor of both the androgen receptor and thyroid receptor and has a potential link to autism (13-15). Members of the JMJD1/JHDM2 family have been shown to demethylate mono-methyl and di-methyl histone H3 (Lys9) (3,8). | | | Catalog # | J7876-46 | | Applications | Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Immunofluorescence: 1:100 | | Western Blot: 1:1000 | | Immunoprecipitation: 1:25 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | | | Form | Supplied as a liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100ug/ml BSA and 50% glycerol. | | Specificity | Detects endogenous levels of JMJD1B protein. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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