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J7876-46 Rabbit Anti-JMJD1B/JHDM2B (Jumonji Domain Containing Protein 1B, JMJD1B, JmjC Domain-containing Histone Demethylation Protein 2B, JHDM2B, KDM3B, 5qNCA, Nuclear Protein 5qNCA)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
Q7LBC6
Isotype
IgG
Grade
Affinity Purified
Applications
IC IF IP WB
Crossreactivity
Hu Mk Mo Rt
Gene ID
51780
Shipping Temp
Blue Ice
Storage Temp
-20°C

The methylation state of lysine residues in histone proteins is major determinant for formation of active and inactive regions of the genome and is crucial for proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain of several proteins has been shown to catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and alpha-ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into seven separate families (3). The JMJD1 (Jumonji domain-containing protein 1) family, also known as JHDM2 (JmjC domain-containing histone demethylation protein 2) family, contains four members: hairless (HR), JMJD1A/JH- DM2A, JMJD1B/JHDM2B, JMJD1C/JHDM2C. Hairless is expressed in the skin and brain and acts as a co-repressor of the thyroid hormone receptor (4-6). Mutations in the hairless gene cause alopecia in both mice and humans (4,5). JMJD1A is expressed in meiotic and post-meiotic male germ cells, contributes to androgen receptor-mediated gene regulation, and is required for spermatogenesis (7-9). It has also been identified as a downstream target of OCT4 and STAT3 and is critical for the regulation of self-renewal in embryonic stem cells (10,11). JMJD1B is a more widely expressed family member and is frequently deleted in myeloid leukemia (12). JMJD1C (also known as TRIP8) is a co-factor of both the androgen receptor and thyroid receptor and has a potential link to autism (13-15). Members of the JMJD1/JHDM2 family have been shown to demethylate mono-methyl and di-methyl histone H3 (Lys9) (3,8).

Applications
Suitable for use inWestern Blot, Immunoprecipitation and Immunofluorescence/ Immunocytochemistry. Other applications not tested.
Recommended Dilution
Immunofluorescence (IF-IC): 1:100 Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:25 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding residues surrounding Pro1254 of human JMJD1B.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of human JMJD1B protein (all three isoforms). Species crossreactivity: mouse, rat and monkey.
References
1. Kubicek, S. et al. (2006) Ernst Schering Res Found Work- shop, 1–27. 2. Lin, W. and Dent, S.Y. (2006) Curr Opin Genet Dev 16, 137–42. 3. Klose, R.J. et al. (2006) Nat Rev Genet 7, 715–27. 4. Cachon-Gonzalez, M.B. et al. (1994) Proc Natl Acad Sci USA 91, 7717–21. 5. Ahmad, W. et al. (1998) Science 279, 720–4. 6. Potter, G.B. et al. (2001) Genes Dev 15, 2687–701. 7. Höög, C. et al. (1991) Mol Reprod Dev 30, 173–81. 8. Yamane, K. et al. (2006) Cell 125, 483–95. 9. Okada, Y. et al. (2007) Nature 450, 119–23. 10. Loh, Y.H. et al. (2007) Genes Dev 21, 2545–57. 11. Ko, S.Y. et al. (2006) Cell Struct Funct 31, 53–62. 12. Hu, Z. et al. (2001) Oncogene 20, 6946–54. 13. Lee, J.W. et al. (1995) Mol Endocrinol 9, 243–54. 14. Wolf, S.S. et al. (2007) Arch Biochem Biophys 460, 56–66. 15. Castermans, D. et al. (2007) Eur J Hum Genet 15, 422–31.
USBio References
No references available
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