Anti -Junctional Adhesion Molecule 4 (JAM4, Immunoglobulin Superfamily Member 5, IGSF5,
Cortical Thymocyte Marker in Xenopus, CTX) (Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
B |
|
| JAM-4, also known as IGSF5, is a 93kD member of the CTX (Cortical Thymocyte Marker in Xenopus) subfamily of the Ig superfamily. It is a type I transmembrane protein with an N-terminal extracellular V-type and C2 type Ig domain separated by a J segment, a transmembrane domain and a cytoplasmic tail with a PDZ binding motif. JAM-4 is expressed in intestinal, renal, and mammary epithelial cells, spermatogonia, and hematopoietic stem cells. It is a cell adhesion molecule that mediates homophilic interaction. JAM-4 also interacts with the intracellular membrane-associated guanylate kinase inverted 1 (MAGI-1). An additional isoform containing a 100 residue insertion between Gln 125 and Asp 126 has been reported. Over the region used as immunogen, mouse JAM-4 shares 82% and 63% amino acid sequence identity with rat and human JAM-4, respectively. | | | Catalog # | J8009-30 | | Applications | Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect recombinant mouse JAM4. The detection limit for rmJAM4 is approximately 5 ng/lane under non-reducing and reducing conditions. In this format, this antibody shows less than 1% crossreactivity with rmJAM-A, rmJAM-B, and rmJAM-C. | | Optimal dilutions should be determined by each laboratory for each application. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile TBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Mouse | | Concentration | As reported | | Form | Supplied as a lyophilized powder from a 0.2um filtered solution in PBS containing 50ug of BSA per 1ug of antibody. Reconstitute with sterile TBS, pH 7.3 (20mM Trizma base, 150mM sodium chloride) containing 0.1% BSA, 40-50% glycerol. If 1ml of buffer is used, the concentration will be 50ug/ml. | | Purity | Purified by mouse JAM4 affinity chromatography and then biotinylated. | | Immunogen | NS0-derived rmJAM4 (aa16-140) | | Specificity | Selected for use as a detection antibody in mouse JAM4 Western Blots. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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