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J8009-30 Sheep Anti-Junctional Adhesion Molecule 4 (JAM4, Immunoglobulin Superfamily Member 5, IGSF5, Cortical Thymocyte Marker in Xenopus, CTX) (Biotin)

Specifications
Clone Type
Polyclonal
Host
Sheep
Source
Mouse
Conjugate
Biotin
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Mo
Accession #
NP_082354
Shipping Temp
Blue Ice
Storage Temp
-20°C

JAM-4, also known as IGSF5, is a 93kD member of the CTX (Cortical Thymocyte Marker in Xenopus) subfamily of the Ig superfamily. It is a type I transmembrane protein with an N-terminal extracellular V-type and C2 type Ig domain separated by a J segment, a transmembrane domain and a cytoplasmic tail with a PDZ binding motif. JAM-4 is expressed in intestinal, renal, and mammary epithelial cells, spermatogonia, and hematopoietic stem cells. It is a cell adhesion molecule that mediates homophilic interaction. JAM-4 also interacts with the intracellular membrane-associated guanylate kinase inverted 1 (MAGI-1). An additional isoform containing a 100 residue insertion between Gln 125 and Asp 126 has been reported. Over the region used as immunogen, mouse JAM-4 shares 82% and 63% amino acid sequence identity with rat and human JAM-4, respectively.

Applications
Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect recombinant mouse JAM4. The detection limit for rmJAM4 is approximately 5 ng/lane under non-reducing and reducing conditions. In this format, this antibody shows less than 1% crossreactivity with rmJAM-A, rmJAM-B, and rmJAM-C. Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
NS0-derived rmJAM4 (aa16-140)
Form
Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Selected for use as a detection antibody in mouse JAM4 Western Blots.
Conjugates
Pricing
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