USBio Logo

K0005-14B Goat Anti-Kallikrein 11 (KLK11, Hippostasin, Trypsin-like serine protease and PRSS20)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Human
Swiss Prot
Q9UBX7
Isotype
IgG
Grade
Affinity Purified
Applications
E IP WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Kallikrein 11 is expressed in brain, skin, ovary and prostate. Increased levels of Kallikrein 11 have been reported in prostate and ovarian carcinoma. It is a serine protease. Kallikrein 11 (KLK11), also known as hippostasin, trypsin-like serine protease and PRSS20, is a member of human tissue kallikrein family.6 Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences, respectively.1-4. Isoform 1 consists of a signal peptide (residues 1-18), a short pro peptide (residues 19-21) and the mature chain (residues 22-250). Isoform 2 is identical to isoform 1, except that a 32 amino acid segment is inserted in isoform 2 before residue1 in isoform 1. Isoform 1 is predominantly expressed in brain whereas isoform 2 is preferentially expressed in prostate. KLK11 is a novel marker for ovarian and prostate cancer carcinomas.7-9 rhKLK11, after being activated by thermolysin, is active against a thioester substrate.

Applications
Suitable for use in ELISA, Western Blot, Immunoprecipitation. Other applications not tested.
Recommended Dilution
Direct ELISA: 0.5-1.0ug/ml with the appropriate secondary reagents to detect human Kallikrein 11. The detection limit for rhKallikrein 11 is ~1.5ng/well.
Western blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect human Kallikrein 11. The detection limit for rhKallikrein 11 is approximately 20ng/lane and 5ng/lane under non-reducing and reducing conditions, respectively.
Immunoprecipitation: Immunoprecipitates rhKallikrein 11 from conditioned media of transfected NS0 cells.
Optimal dilutions to be determined by the researcher.
Recommended Positive Control Tissue
Ovarian tumor
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
NS0-derived, purified recombinant, human Kallikrein 11, aa 22-250
Form
Supplied as a liquid in 0.2um filtered solution in PBS with 5% trehalose.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Kallikrein 11 in direct ELISA and Western Blot. 10% crossreactivity with recombinant, human Kallikrein 3 and less than 1% crossreactivity with recombinant, human Kallikrein 5.
References
1. Yoshida, S. et al., 1998, Biochim. Biophys. Acta 1399:225 - 228.|2. Yousef, G.M. et al., 2000,Genomics 63:88 - 96.|3. Mitsui, S. et al., 2000, Biochem. Biophys. Res. Commun. 272:205 - 211.|4. Gan, L. et al., 2000, Gene 257:119 - 130.|5. Edwards, K.M. et al., 1999, J. Biol. Chem. 274:30486 - 30473.|6. Yousef, G.M. and E.P. Diamandis, 2001, Endocrine Rev. 22:184 - 204.|7. Diamandis, E.P. et al., 2002, Cancer Res. 62:295 - 300.|8. Nakamura, T. et al., 2003, Urology 61:1042 - 1046.|9. Borgono, C.A. et al., 2003, Int. J. Cancer 106:605 - 610.
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved