| Kallikrein 11 is expressed in brain, skin, ovary and prostate. Increased levels of Kallikrein 11 have been reported in prostate and ovarian carcinoma. It is a serine protease. Kallikrein 11 (KLK11), also known as hippostasin, trypsin-like serine protease and PRSS20, is a member of human tissue kallikrein family.6 Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences, respectively.1-4. Isoform 1 consists of a signal peptide (residues 1-18), a short pro peptide (residues 19-21) and the mature chain |
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| (residues 22-250). Isoform 2 is identical to isoform 1, except that a 32 amino acid segment is inserted in isoform 2 before residue1 in isoform 1. Isoform 1 is predominantly expressed in brain whereas isoform 2 is preferentially expressed in prostate. KLK11 is a novel marker for ovarian and prostate cancer carcinomas.7-9 rhKLK11, after being activated by thermolysin, is active against a thioester substrate. |
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| Catalog # | K0005-14B |
| Applications | Suitable for use in ELISA, Western Blot, Immunoprecipitation. Other applications not tested. |
| Recommended Dilution | Direct ELISA: 0.5-1.0ug/ml with the appropriate secondary reagents to detect human Kallikrein 11. The detection limit for rhKallikrein 11 is ~1.5ng/well. |
| Western blot | 0.1-0.2ug/ml with the appropriate secondary reagents to detect human Kallikrein 11. The detection limit for rhKallikrein 11 is approximately 20ng/lane and 5ng/lane under |
| non-reducing and reducing conditions, respectively. |
| Immunoprecipitation | Immunoprecipitates rhKallikrein 11 from conditioned media of transfected NS0 cells. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Positive Control Tissue | Ovarian tumor |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Human |
| Concentration | ~0.1mg/ml |
| Form | Supplied as a liquid in 0.2um filtered solution in PBS with 5% trehalose. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | NS0-derived, purified recombinant, human Kallikrein 11, aa 22-250 |
| Specificity | Recognizes human Kallikrein 11 in direct ELISA and Western Blot. 10% crossreactivity with recombinant, human Kallikrein 3 and less than 1% crossreactivity with recombinant, human Kallikrein 5. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
