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You are here:Home » Antibodies » Abs to Kallikrein » Anti -Kallikrein 11 (KLK11, Hippostasin, Trypsin-like serine protease and PRSS20)

Anti -Kallikrein 11 (KLK11, Hippostasin, Trypsin-like serine protease and PRSS20)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IP
Kallikrein 11 is expressed in brain, skin, ovary and prostate. Increased levels of Kallikrein 11 have been reported in prostate and ovarian carcinoma. It is a serine protease. Kallikrein 11 (KLK11), also known as hippostasin, trypsin-like serine protease and PRSS20, is a member of human tissue kallikrein family.6 Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences, respectively.1-4. Isoform 1 consists of a signal peptide (residues 1-18), a short pro peptide (residues 19-21) and the mature chain
(residues 22-250). Isoform 2 is identical to isoform 1, except that a 32 amino acid segment is inserted in isoform 2 before residue1 in isoform 1. Isoform 1 is predominantly expressed in brain whereas isoform 2 is preferentially expressed in prostate. KLK11 is a novel marker for ovarian and prostate cancer carcinomas.7-9 rhKLK11, after being activated by thermolysin, is active against a thioester substrate.
Catalog #K0005-14B
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation. Other applications not tested.
Recommended DilutionDirect ELISA: 0.5-1.0ug/ml with the appropriate secondary reagents to detect human Kallikrein 11. The detection limit for rhKallikrein 11 is ~1.5ng/well.
Western blot 0.1-0.2ug/ml with the appropriate secondary reagents to detect human Kallikrein 11. The detection limit for rhKallikrein 11 is approximately 20ng/lane and 5ng/lane under
non-reducing and reducing conditions, respectively.
Immunoprecipitation Immunoprecipitates rhKallikrein 11 from conditioned media of transfected NS0 cells.
Optimal dilutions to be determined by the researcher.
Recommended Positive Control TissueOvarian tumor
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in 0.2um filtered solution in PBS with 5% trehalose.
PurityPurified by immunoaffinity chromatography.
ImmunogenNS0-derived, purified recombinant, human Kallikrein 11, aa 22-250
SpecificityRecognizes human Kallikrein 11 in direct ELISA and Western Blot. 10% crossreactivity with recombinant, human Kallikrein 3 and less than 1% crossreactivity with recombinant, human Kallikrein 5.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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