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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IP

Kallikrein 15, encoded by the KLK15 gene, is a member of the tissue Kallikrein family of Serine proteases. It is a secreted protein found in prostate, colon, thyroid tissues, breast milk and seminal plasma. Whether it has a trypsin-like activity or not remains controversial. Several splice forms exist. The 250 aa rhKLK15 has a 10-His tag at the C-terminus. It predicts a molecular mass of 28kD and migrates as a 39kD band in SDS-PAGE under reducing conditions. The amino acid sequence of human KLK15 is 99%, 77% and 77% identical to that of chimpanzee, canine and mouse, respectively.
Catalog #	K0005-18C
Applications	Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human KLK15. The detection limit for rhKLK15 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: This antibody has been used to immunoprecipitate rhKLK15 from conditioned media of transfected NS0 cells. The recovered rhKLK15 can be detected by Western blot analysis using a monoclonal antibody.
	Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human KLK15. The detection limit for rhKLK15 is approximately 0.5 ng/well.
	Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	As reported
Form	Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
Purity	Purified by human KLK15 affinity chromatography.
Immunogen	NS0-derived recombinant human KLK15 isoform 1 (aa17-256)
Specificity	Selected for its ability to recognize human KLK15 in direct ELISAs and Western Blots. In these formats, this antibody shows less than 1% crossreactivity with rhKLK2, rhKLK8, and rhKLK11.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.