kelch-like protein KLHL6, kelch-like 6, FLJ00029, kelch-like 6 (Drosophila), KLHL6
Applications
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
Peptide ELISA Titer: 1:16,000 Western Blot: 1-3ug/ml ~80kD band observed in Human Tonsil lysates and in lysates of cell lines NIH3T3 (calculated MW of 70.4kD according to Human NP_569713.2 and of 70.2kD according to Mouse NP_899246.2). Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-SERTKPRMHEFQSE, from the internal region of human KLHL6 (NP_569713.2).
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by peptide affinity chromatography.
Specificity
Recognizes human KLHL6. Species crossreactivity: mouse. Species sequence homology: rat, canine, bovine.
References
1. Gupta-Rossi N, Storck S, Griebel PJ, Reynaud CA, Weill JC, Dahan A, Specific over-expression of deltex and a new Kelch-like protein in human germinal center B cells. Molecular immunology 2003 Mar 39 (13): 791-9.