Suitable for use in Western Blot, ELISA and RIA. May be used in a competitive inhibition assay to measure keratan sulfate levels. Other applications not tested.

Western Blot: 1:1000-1:4000 ELISA: 1:5000-1:20,000 RIA: 1:30,000-1:500,000 Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Human articular cartilage proteoglycan digested with chondroitinase ABC.

Supplied as a liquid. No preservative added.

Ascites.

Recognizes 2-3 disaccharide units on both skeletal (type II) and corneal (type I) keratan sulfate chains either in isolated or proteoglycan-bound form. The epitope is resistant to proteolytic or alkaline cleavages and is partially sensitive to keratanase or other endo-b-galactosidase treatment. Species Crossreactivity: human, bovine, sheep, porcine, canine and mouse (adult). No crossreactivity with chicken limb bud proteoglycans, dermatan sulfate proteoglycans of cartilage and proteoglycans of rat chondrosarcomas.