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K0199-70 Mouse Anti-Keratin, Epithelial

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P05787
Isotype
IgG1
Clone Number
AE3
Grade
Purified
Applications
IHC WB
Crossreactivity
Bo Hu Mk Mo Rb Rt
Accession #
NM_002273.2
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications
Suitable for use in Immunohistochemistry (unfixed frozen sections and formalin-fixed paraffin embedded tissues) and Western Blot. Other applications not tested.
Recommended Dilution
Immunohistochemistry (paraffin): antigen retrieval using HIER or trypsin treatment. Optimal dilutions to be determined by the researcher.
Positive Control
MCF-7, RT112 and A432 cell lines
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human epidermal cells
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified
Specificity
Recognizes most of the basic (Type II) keratins. It is broadly reactive and stains positively almost all epithelia and their neoplasms. In Western blotting, this clone recognizes the 65 to 67, 64, 59, 58, 56 and 52 kDa human cytokeratins of the basic subfamily. Species Crossreactivity: human, rabbit, mouse, rat, bovine, chicken and monkey.
References
Mikaelian, I. et al. (2004). Antibodies That Label Paraffin-Embedded Mouse Tissues: A Collaborative Endeavor. Toxicology Pathology 32:181-191.|Clausen, H. et al. (1986). Differentiation-dependent expression of keratins in human oral epithelia. J. Invest. Dermatol. 86:249-254.|Sun, T-T, et al. (1985). Monoclonal antibody studies of mammalian epithelial keratins: a review. Ann. N.Y. Acad. Sci. 455:307-329.|Spagnolo, D.V. et al. (1985). Monoclonal anti-keratin (AE1) reactivity in routinely processed tissue from 166 human neoplasms. Am. J. Clin. Path. 84:697-704. Battifora, H. et al. (1984). Antikeratin antibodies in tumor diagnosis. Distinction between seminoma and embryonal carcinoma. Cancer 54:843-848.|Eichner, R et al. (1984). Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression. J Cell Biol. 98:1388-1396.|Shi, S-R. et al. (1984). Immunohistochemical study of nasopharyngeal carcinoma using monoclonal keratin antibodies. Am. J. Pathol. 117:53-63.|Weiss, R. A. et al. (1984). Monoclonal antibody analysis of keratin expression in epidermal diseases: a 48- and 56-kdalton keratin as molecular markers for hyperproliferative keratinocytes. J. Cell Biol. 98:1397-1406.|Sun, T-T. et al. (1983). Keratin classes: molecular markers for different types of epithelial differentiation. J. Invest. Dermatol. 81:109s-115s.|Woodcock-Mitchell, J. et al. (1982). Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J. Cell Biol. 95:580-88.|Sun, T-T and Green, H. (1978). Immunofluorescent staining of keratin fibers in cultured cells. Cell 14:469-476.
USBio References
No references available
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