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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified B IH

Catalog #	K0199-70
Applications	Suitable for use in Immunohistochemistry (unfixed frozen sections and formalin-fixed paraffin embedded tissues) and Western Blot. Other applications not tested.
Recommended Dilution	Immunohistochemistry (paraffin): We recommend antigen retrieval using HIER or trypsin treatment.
	Optimal dilutions to be determined by the researcher.
Positive Control	MCF-7, RT112 and A432 cell lines
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type	Monoclonal
Isotype	IgG1
Clone No	AE3
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, 0.1% sodium azide.
Purity	Purified IgG
Immunogen	Human epidermal keratin
Specificity	Recognizes most of the basic (Type II) keratins. It is broadly reactive and stains positively almost all epithelia and their neoplasms. In Western blotting, this clone recognizes the 65 to 67, 64, 59, 58, 56 and 52 kDa human cytokeratins of the basic subfamily. Species Reactivity: human, rabbit, mouse, rat, bovine , chicken and monkey.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.