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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Keratin (Cytokeratin Type I and II), pan, multiple clones (BSA & Azide Free)

Anti -Keratin (Cytokeratin Type I and II), pan, multiple clones (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH IF
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights of 56.5, 55, 51, 50, 50 (doublet), 48, 46, 45 and 40kD. The basic keratins have molecular weights of 65-67, 64, 59, 58, 56 and 52kD. Members of acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with cell type, differentiation stauts, and environment. Many studies have shown the usefulness of keratins as markerd in cancer research and tumor diagnosis.
Catalog #K0199-90X
ApplicationsSuitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (Formalin/paraffin): 1:25 for 30 min at RT. For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 10 min at 37°C, or Protease XXV at 1mg/ml PBS for 5 minutes at 37°C.
Optimal dilutions to be determined by the researcher.
SourceAscites
Positive ControlSkin and lung carcinomas
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.368 + 0.N.370
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, before the addition of glycerol to 40%. No preservatives and no stablizing proteins added. Available with BSA and azide. See K0199-90.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman epidermal keratin. Cellular Localization: Cytoplasmic.
SpecificityRecognizes acidic keratins at 56.5, 50 (doublet), 48 and 40kD. Recognizes basic keratins at 65-67, 64, 59, 58, 56 and 52kD. Reacts with keratinized (56.5/65-67) and corneal (55/64) epidermis, stratified squamous epithelia of internal organs (51/59), stratified epithelia (50/58), hyperproliferative keratinocytes
(48/56) and simple epithelia (45/52 and 46/54). Anti pan-keratin antibody cocktail differentiates epithelial from non-epithelial tumors. Species CrossreactivityHuman, monkey, bovine, rabbit, mouse, rat and chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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