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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IC

Catalog #	L1220-08
Applications	Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution	Immunoblot Analysis: 0.5-2ug/ml of this lot detected Lamin A/C in 20ug of RIPA lysates from A431 cells.
	Immunocytochemistry: This antibody has been reported by an independent laboratory to detect Lamin A/C in human endothelial cells.
	Optimal dilutions to be determined by the researcher.
Included Positive Antigen Control	Non-stimulated A431 lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG1
Clone No	5H29
Host	Mouse
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a liquid in 50% storage buffer (20mM sodium phosphate, pH 7.5, 0.15M sodium chloride, 1mg/ml BSA, 0.09% sodium azide) and 50% glycerol.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Peptide from human lamin A/C corresponding to aa398 to 490.
Specificity	Peptide from human lamin A/C corresponding to aa398-490. Recognizes Lamin A, Mr 74kD and Lamin C, Mr 65kD. Species Crossreactivity: Human, mouse, rat, chicken and canine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.